Effect of staphylococcal lipoteichoic acid on differentiation of RAW264.7 cells into osteoclasts

• Published in: Zhongguo xiu fu chong jian wai ke za zhi 2017-02, Vol.31 (2), p.180-184
• Main Authors: Ren, Lirong, Xu, Yongqing, Wang, Hai, He, Xiaoqing, Song, Muguo, Chen, Xueqiu
• Format: Article
• Language: Chinese
• Subjects: Bone Resorption; Cell Differentiation - drug effects; Cell Line; Humans; Lipopolysaccharides - pharmacology; Osteoclasts - drug effects; Osteoclasts - physiology; RANK Ligand; Staphylococcus - chemistry; Teichoic Acids - pharmacology
• ISSN: 1002-1892
• DOI: 10.7507/1002-1892.201610077

To investigate the effect of lipoteichoic acid (LTA-sa) on RAW264.7 cells differentiation into osteoclasts. RAW264.7 cells were cultured with LTA-sa of 100 ng/mL (group A), LTA-sa of 200 ng/mL (group B), LTA-sa of 400 ng/mL (group C), receptor activator of nuclear factor κB (NF-κB) ligand (RANKL) of 100 ng/mL as positive control (group D), and equal volume of PBS as blank control (group E) respectively for 5 days. And then, tartrate resistant acid phosphatase staining (TRAP) was used to detect the formation of osteoclast-like cells, Image-Pro Plus 6.0 software to measure the areas of bone resorption pits in Corning Osteo Assay Surface (COAS) wells, and MTT assay to observe the proliferation activity of RAW264.7 cells in group A, B, C, and E. After cultured for 5 days, the formation of osteoclast-like cells and bone resorption pits were observed in all groups. The number of osteoclast-like cells and the area of bone resorption pits in groups A, B, C, and D were more than those in group E. And with the increase