Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress

To thoroughly investigate the time-course changes of several commonly used markers of oxidative stress by performing serial measurements during a 24-h period after an acute bout of strenuous cardiovascular exercise. Eleven untrained men performed two trials. In the experimental trial, the subjects e...

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Published in:Medicine and science in sports and exercise 2007-07, Vol.39 (7), p.1107-1113
Main Authors: MICHAILIDIS, Yiannis, JAMURTAS, Athanasios Z, NIKOLAIDIS, Michalis G, FATOUROS, Ioannis G, KOUTEDAKIS, Yiannis, PAPASSOTIRIOU, Ioannis, KOURETAS, Dimitris
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Catalase - analysis
Exercise - physiology
Fundamental and applied biological sciences. Psychology
Glutathione - analysis
Glutathione - blood
Greece
Hematologic Tests - utilization
Humans
Male
Oxidative Stress - physiology
Protein Carbonylation
Research Design
Thiobarbituric Acid Reactive Substances - analysis
Time Factors
Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports
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Summary:To thoroughly investigate the time-course changes of several commonly used markers of oxidative stress by performing serial measurements during a 24-h period after an acute bout of strenuous cardiovascular exercise. Eleven untrained men performed two trials. In the experimental trial, the subjects exercised for 45 min at 70-75% VO2max and then at 90% VO2max to exhaustion on a treadmill; in the control trial, the subjects remained at rest. Blood samples were drawn before and after exercise (immediately after exercise and at 0.5, 1, 2, 3, 4, 5, 6, 8, 10, and 24 h). Reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG, thiobarbituric acid-reactive substances (TBARS), protein carbonyls, catalase activity, and total antioxidant capacity (TAC) were determined. The time to lowest concentration after exercise was 1.7 +/- 0.7 h (mean +/- SD) for GSH/GSSG, and the time to highest concentration after exercise was 1.2 +/- 0.6 h for TBARS, 4.4 +/- 0.5 h for protein carbonyls, 0.5 +/- 0.4h for catalase, and 2.2 +/- 0.9 h for TAC. The greatest change after exercise was -74 +/- 9% for GSH/GSSG, 129 +/- 29% for TBARS, 135 +/- 53% for protein carbonyls, 51 +/- 16% for catalase, and 24 +/- 10% for TAC. There is no best time point applying to all markers for collecting blood samples after aerobic exercise. The optimum postexercise time points for blood collection in untrained individuals are immediately after exercise for catalase, 1 h for TBARS, 2 h for TAC, GSH, and GSSG, and 4 h after exercise for protein carbonyls.
ISSN:0195-9131
1530-0315
DOI:10.1249/01.mss.0b013e318053e7ba