DEVELOPMENT OF AN ENZYME-LINKED IMMUNOELECTROTRANSFER BLOT (EITB) ASSAY USING TWO BACULOVIRUS EXPRESSED RECOMBINANT ANTIGENS FOR DIAGNOSIS OF TAENIA SOLIUM TAENIASIS

• Published in: The Journal of parasitology 2007-04, Vol.93 (2), p.409-417
• Main Authors: Levine, Min Z, Lewis, Melissa M, Rodriquez, Silvia, Jimenez, Juan A, Khan, Azra, Lin, Sehching, Garcia, Hector H, Gonzales, Armando E, Gilman, Robert H, Tsang, Victor C. W
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Helminth - blood; Antigens; Antigens, Helminth - genetics; Antigens, Helminth - immunology; Baculoviridae; Baculoviridae - genetics; Baculovirus; Biological and medical sciences; blood serum; Cell culture; Coproantigens; Cysticercosis; Cysts; Developing countries; Diagnosis; diagnostic techniques; disease diagnosis; enzyme immunoassays; Enzymes; Ethanol; excretory-secretory products; Fundamental and applied biological sciences. Psychology; gene expression; General aspects; General aspects and techniques. Study of several systematic groups. Models; Hogs; Humans; immunoblotting; Immunoblotting - methods; Infections; Insect cells; Insects; Invertebrates; Laboratory tests; LDCs; Medical diagnostic tests; Molecular weight; new methods; Parasites; Parasitic diseases; Patients; Proteins; recombinant antigens; Recombinant Proteins - immunology; Sensitivity; Sensitivity and Specificity; serodiagnosis; Serology; Taenia solium; Taenia solium - immunology; Taeniasis; Taeniasis - diagnosis; Taeniasis - immunology; Tapeworms; THERAPEUTICS-DIAGNOSTICS; Tropical diseases; Worms
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-938R.1

Taeniasis diagnosis is an important step in the control and elimination of both cysticercosis and taeniasis. We report the development of 2 serological taeniasis diagnostic tests using recombinant antigens rES33 and rES38 expressed by baculovirus in insect cells in an EITB format. In laboratory testing with defined sera from nonendemic areas, rES33 has a sensitivity of 98% (n = 167) and a specificity of 99% (n = 310) (J index: 0.97); rES38 has a sensitivity of 99% (n = 146) and a specificity of 97% (n = 275) (J index: 0.96). Independent field testing in Peru showed 97% (n = 203) of the taeniasis sera were positive with rES33, and 100% of the nontaeniasis sera (n = 272) were negative with rES33; 98% (n = 198) of taeniasis sera were positive with rES38, and 91% (n = 274) of the nontaeniasis sera were negative with rES38. Among the Peruvian sera tested, 17 of 26 Peruvian Taenia saginata sera were false positive with rES38 test. Both tests were also examined with cysticercosis sera, with a positive rate ranging from 21% to 46%. rES33 and rES38 tests offer sensitive and specific diagnosis of taeniasis and easy sample collection through finger sticks that can be used in large-scale studies. They are currently being used in cysticercosis elimination programs in Peru.