Catalase encapsulated in mesoporous silica and its performance

Catalase was confined in a nanospace of about 7 nm in diameter in mesoporous silica (FSM; folded-sheet mesoporous material), forming a catalase-FSM conjugate. That the enzyme was encapsulated in the pores of mesoporous materials was proven by the direct visualization technique. The catalase-FSM show...

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Bibliographic Details
Published in:Biochemical engineering journal 2009-05, Vol.44 (2), p.167-173
Main Authors: Itoh, Tetsuji, Ishii, Ryo, Matsuura, Shun-ichi, Hamakawa, Satoshi, Hanaoka, Takaaki, Tsunoda, Tatsuo, Mizuguchi, Junko, Mizukami, Fujio
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Catalase
Fundamental and applied biological sciences. Psychology
Mesoporous silica
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Summary:Catalase was confined in a nanospace of about 7 nm in diameter in mesoporous silica (FSM; folded-sheet mesoporous material), forming a catalase-FSM conjugate. That the enzyme was encapsulated in the pores of mesoporous materials was proven by the direct visualization technique. The catalase-FSM showed not only an activity similar to that of the native catalase in the decomposition of H 2O 2 but also much higher stability: specifically, the catalase immobilized in FSM still retained its original activity after being applied 50 times to the decomposition, whereas the native lost its activity after 20 times recycling. Furthermore, the catalase-FSM showed catalase activity even in an organic media in which the native catalase had almost no catalytic activity.
ISSN:1369-703X
1873-295X
DOI:10.1016/j.bej.2008.11.015