Isolation of Rh-TIP1;1, an aquaporin gene and its expression in rose flowers in response to ethylene and water deficit

Flower opening is a process that involves water uptake by petal cells, during which aquaporins serve as the channels for water transport across the cell membranes. In this study, we isolated a TIP type aquaporin gene, Rh-TIP1;1, from rose ( Rosa hybrida cv. Samantha). The Rh-TIP1;1 transcript is 114...

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Bibliographic Details
Published in:Postharvest biology and technology 2009-03, Vol.51 (3), p.407-413
Main Authors: Xue, Jingqi, Yang, Feng, Gao, Junping
Format: Article
Language:English
Subjects:
1-methylcyclopropene
amino acid sequences
Aquaporin
aquaporins
Biological and medical sciences
cell membranes
cut flowers
Cut roses
Ethylene
floriculture crops
Flower opening
flowering
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
gene expression
gene expression regulation
messenger RNA
molecular sequence data
molecular weight
nucleotide sequences
plant growth substances
postharvest physiology
postharvest treatment
protein synthesis
Rh-TIP1
1
Rosa
temporal variation
Water deficit
water stress
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Summary:Flower opening is a process that involves water uptake by petal cells, during which aquaporins serve as the channels for water transport across the cell membranes. In this study, we isolated a TIP type aquaporin gene, Rh-TIP1;1, from rose ( Rosa hybrida cv. Samantha). The Rh-TIP1;1 transcript is 1142 bp in length and encodes a deduced protein of 253 amino acids. Through a prokaryotic expression system, we obtained a predominant ∼32 kDa protein band which is consistent with the predicted molecular weight of Rh-TIP1;1. Rh-TIP1;1 was mainly expressed in petals among all floral tissues at the fully opened bud stage. Its expression was maintained at a high level during rapid flower opening, and decreased when the flowers were fully opened. Ethylene and water deficit both decreased the expression of Rh-TIP1;1. Flowers treated with 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, maintained the transcript at a high level for longer than untreated controls. These results indicated that the effects of ethylene and water deficit on flower opening may be related to their influence on the expression of the Rh-TIP1;1 gene.
ISSN:0925-5214
1873-2356
DOI:10.1016/j.postharvbio.2008.08.011