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Isolation of Rh-TIP1;1, an aquaporin gene and its expression in rose flowers in response to ethylene and water deficit
Flower opening is a process that involves water uptake by petal cells, during which aquaporins serve as the channels for water transport across the cell membranes. In this study, we isolated a TIP type aquaporin gene, Rh-TIP1;1, from rose ( Rosa hybrida cv. Samantha). The Rh-TIP1;1 transcript is 114...
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| Published in: | Postharvest biology and technology 2009-03, Vol.51 (3), p.407-413 |
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| cited_by | cdi_FETCH-LOGICAL-c472t-f0997eed901605eee9c37abca82ace8e991d3a98898cdeec85f5e38b68e51b443 |
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| cites | cdi_FETCH-LOGICAL-c472t-f0997eed901605eee9c37abca82ace8e991d3a98898cdeec85f5e38b68e51b443 |
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| container_title | Postharvest biology and technology |
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| creator | Xue, Jingqi Yang, Feng Gao, Junping |
| description | Flower opening is a process that involves water uptake by petal cells, during which aquaporins serve as the channels for water transport across the cell membranes. In this study, we isolated a TIP type aquaporin gene,
Rh-TIP1;1, from rose (
Rosa hybrida cv. Samantha). The
Rh-TIP1;1 transcript is 1142
bp in length and encodes a deduced protein of 253 amino acids. Through a prokaryotic expression system, we obtained a predominant ∼32
kDa protein band which is consistent with the predicted molecular weight of Rh-TIP1;1.
Rh-TIP1;1 was mainly expressed in petals among all floral tissues at the fully opened bud stage. Its expression was maintained at a high level during rapid flower opening, and decreased when the flowers were fully opened. Ethylene and water deficit both decreased the expression of
Rh-TIP1;1. Flowers treated with 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, maintained the transcript at a high level for longer than untreated controls. These results indicated that the effects of ethylene and water deficit on flower opening may be related to their influence on the expression of the
Rh-TIP1;1 gene. |
| doi_str_mv | 10.1016/j.postharvbio.2008.08.011 |
| format | article |
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Rh-TIP1;1, from rose (
Rosa hybrida cv. Samantha). The
Rh-TIP1;1 transcript is 1142
bp in length and encodes a deduced protein of 253 amino acids. Through a prokaryotic expression system, we obtained a predominant ∼32
kDa protein band which is consistent with the predicted molecular weight of Rh-TIP1;1.
Rh-TIP1;1 was mainly expressed in petals among all floral tissues at the fully opened bud stage. Its expression was maintained at a high level during rapid flower opening, and decreased when the flowers were fully opened. Ethylene and water deficit both decreased the expression of
Rh-TIP1;1. Flowers treated with 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, maintained the transcript at a high level for longer than untreated controls. These results indicated that the effects of ethylene and water deficit on flower opening may be related to their influence on the expression of the
Rh-TIP1;1 gene.</description><identifier>ISSN: 0925-5214</identifier><identifier>EISSN: 1873-2356</identifier><identifier>DOI: 10.1016/j.postharvbio.2008.08.011</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>1-methylcyclopropene ; amino acid sequences ; Aquaporin ; aquaporins ; Biological and medical sciences ; cell membranes ; cut flowers ; Cut roses ; Ethylene ; floriculture crops ; Flower opening ; flowering ; Food industries ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; gene expression ; gene expression regulation ; messenger RNA ; molecular sequence data ; molecular weight ; nucleotide sequences ; plant growth substances ; postharvest physiology ; postharvest treatment ; protein synthesis ; Rh-TIP1 ;1 ; Rosa ; temporal variation ; Water deficit ; water stress</subject><ispartof>Postharvest biology and technology, 2009-03, Vol.51 (3), p.407-413</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-f0997eed901605eee9c37abca82ace8e991d3a98898cdeec85f5e38b68e51b443</citedby><cites>FETCH-LOGICAL-c472t-f0997eed901605eee9c37abca82ace8e991d3a98898cdeec85f5e38b68e51b443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21247376$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Jingqi</creatorcontrib><creatorcontrib>Yang, Feng</creatorcontrib><creatorcontrib>Gao, Junping</creatorcontrib><title>Isolation of Rh-TIP1;1, an aquaporin gene and its expression in rose flowers in response to ethylene and water deficit</title><title>Postharvest biology and technology</title><description>Flower opening is a process that involves water uptake by petal cells, during which aquaporins serve as the channels for water transport across the cell membranes. In this study, we isolated a TIP type aquaporin gene,
Rh-TIP1;1, from rose (
Rosa hybrida cv. Samantha). The
Rh-TIP1;1 transcript is 1142
bp in length and encodes a deduced protein of 253 amino acids. Through a prokaryotic expression system, we obtained a predominant ∼32
kDa protein band which is consistent with the predicted molecular weight of Rh-TIP1;1.
Rh-TIP1;1 was mainly expressed in petals among all floral tissues at the fully opened bud stage. Its expression was maintained at a high level during rapid flower opening, and decreased when the flowers were fully opened. Ethylene and water deficit both decreased the expression of
Rh-TIP1;1. Flowers treated with 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, maintained the transcript at a high level for longer than untreated controls. These results indicated that the effects of ethylene and water deficit on flower opening may be related to their influence on the expression of the
Rh-TIP1;1 gene.</description><subject>1-methylcyclopropene</subject><subject>amino acid sequences</subject><subject>Aquaporin</subject><subject>aquaporins</subject><subject>Biological and medical sciences</subject><subject>cell membranes</subject><subject>cut flowers</subject><subject>Cut roses</subject><subject>Ethylene</subject><subject>floriculture crops</subject><subject>Flower opening</subject><subject>flowering</subject><subject>Food industries</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>gene expression regulation</subject><subject>messenger RNA</subject><subject>molecular sequence data</subject><subject>molecular weight</subject><subject>nucleotide sequences</subject><subject>plant growth substances</subject><subject>postharvest physiology</subject><subject>postharvest treatment</subject><subject>protein synthesis</subject><subject>Rh-TIP1 ;1</subject><subject>Rosa</subject><subject>temporal variation</subject><subject>Water deficit</subject><subject>water stress</subject><issn>0925-5214</issn><issn>1873-2356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkE1v1DAQhi1EJZbCb8Ac4EQW24kTW5zQCspKlaj6cba8zrjrVRqnHu-W_vs6bEEckUYazfh5ZzwvIe85W3LG28-75RQxb206bEJcCsbUcg7OX5AFV11diVq2L8mCaSErKXjzirxG3DHGpJRqQQ5rjIPNIY40enq5ra7XF_wL_0TtSO393k4xhZHewgil09OQkcKvKQHiLClPKSJQP8QHSPi7BpziWHo5Usjbx-GP9MFmSLQHH1zIb8iJtwPC2-d8Sm6-f7te_ajOf56tV1_PK9d0Ileead0B9LqcyiQAaFd3duOsEtaBAq15X1utlFauB3BKegm12rQKJN80TX1KPh7nTine7wGzuQvoYBjsCHGPRrCmUbLVBdRH0JWDMIE3Uwp3Nj0azszstNmZf5w2s9NmDs6L9sPzEovODj7Z0QX8O0Bw0XR11xbu3ZHzNhp7mwpzcyUYrxmXSndSFWJ1JKB4cgiQDLoAo4M-JHDZ9DH8x3-eAN2TpZc</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Xue, Jingqi</creator><creator>Yang, Feng</creator><creator>Gao, Junping</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20090301</creationdate><title>Isolation of Rh-TIP1;1, an aquaporin gene and its expression in rose flowers in response to ethylene and water deficit</title><author>Xue, Jingqi ; Yang, Feng ; Gao, Junping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-f0997eed901605eee9c37abca82ace8e991d3a98898cdeec85f5e38b68e51b443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>1-methylcyclopropene</topic><topic>amino acid sequences</topic><topic>Aquaporin</topic><topic>aquaporins</topic><topic>Biological and medical sciences</topic><topic>cell membranes</topic><topic>cut flowers</topic><topic>Cut roses</topic><topic>Ethylene</topic><topic>floriculture crops</topic><topic>Flower opening</topic><topic>flowering</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>gene expression regulation</topic><topic>messenger RNA</topic><topic>molecular sequence data</topic><topic>molecular weight</topic><topic>nucleotide sequences</topic><topic>plant growth substances</topic><topic>postharvest physiology</topic><topic>postharvest treatment</topic><topic>protein synthesis</topic><topic>Rh-TIP1 ;1</topic><topic>Rosa</topic><topic>temporal variation</topic><topic>Water deficit</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xue, Jingqi</creatorcontrib><creatorcontrib>Yang, Feng</creatorcontrib><creatorcontrib>Gao, Junping</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Postharvest biology and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xue, Jingqi</au><au>Yang, Feng</au><au>Gao, Junping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Rh-TIP1;1, an aquaporin gene and its expression in rose flowers in response to ethylene and water deficit</atitle><jtitle>Postharvest biology and technology</jtitle><date>2009-03-01</date><risdate>2009</risdate><volume>51</volume><issue>3</issue><spage>407</spage><epage>413</epage><pages>407-413</pages><issn>0925-5214</issn><eissn>1873-2356</eissn><abstract>Flower opening is a process that involves water uptake by petal cells, during which aquaporins serve as the channels for water transport across the cell membranes. In this study, we isolated a TIP type aquaporin gene,
Rh-TIP1;1, from rose (
Rosa hybrida cv. Samantha). The
Rh-TIP1;1 transcript is 1142
bp in length and encodes a deduced protein of 253 amino acids. Through a prokaryotic expression system, we obtained a predominant ∼32
kDa protein band which is consistent with the predicted molecular weight of Rh-TIP1;1.
Rh-TIP1;1 was mainly expressed in petals among all floral tissues at the fully opened bud stage. Its expression was maintained at a high level during rapid flower opening, and decreased when the flowers were fully opened. Ethylene and water deficit both decreased the expression of
Rh-TIP1;1. Flowers treated with 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, maintained the transcript at a high level for longer than untreated controls. These results indicated that the effects of ethylene and water deficit on flower opening may be related to their influence on the expression of the
Rh-TIP1;1 gene.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><doi>10.1016/j.postharvbio.2008.08.011</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Postharvest biology and technology, 2009-03, Vol.51 (3), p.407-413 |
| issn | 0925-5214 1873-2356 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | 1-methylcyclopropene amino acid sequences Aquaporin aquaporins Biological and medical sciences cell membranes cut flowers Cut roses Ethylene floriculture crops Flower opening flowering Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology gene expression gene expression regulation messenger RNA molecular sequence data molecular weight nucleotide sequences plant growth substances postharvest physiology postharvest treatment protein synthesis Rh-TIP1 1 Rosa temporal variation Water deficit water stress |
| title | Isolation of Rh-TIP1;1, an aquaporin gene and its expression in rose flowers in response to ethylene and water deficit |
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