Isolation and characterization of polymorphic microsatellite loci in the razor clam Ensis siliqua

Five polymorphic microsatellite loci in the razor clam Ensis siliqua are described. A collection consisting of 34 individuals from Finisterre, Spain, was analysed. Loci were isolated from the sequences of intersimple sequence repeat (ISSR) markers. Detailed analysis of 42 ISSR markers led to the des...

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Bibliographic Details
Published in:Molecular ecology notes 2007-03, Vol.7 (2), p.221-222
Main Authors: VARELA, M.A, GONZÁLEZ-TIZÓN, A, FRANCISCO-CANDEIRA, M, MARTÍNEZ-LAGE, A
Format: Article
Language:English
Subjects:
Ensis siliqua
ISSR
microsatellite repeats
microsatellites
razor clam
Siliqua patula
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Summary:Five polymorphic microsatellite loci in the razor clam Ensis siliqua are described. A collection consisting of 34 individuals from Finisterre, Spain, was analysed. Loci were isolated from the sequences of intersimple sequence repeat (ISSR) markers. Detailed analysis of 42 ISSR markers led to the design of 16 primer pairs. Five of these yielded consistent and polymorphic products. The number of alleles ranged from five to 23 per locus with the observed heterozygosity ranging from 0.46 to 0.94. Linkage equilibrium was observed in all loci and three of them showed significant deviations from Hardy-Weinberg equilibrium.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2006.01423.x