Isolation and characterization of 13 polymorphic microsatellite loci for the Florida pompano, Trachinotus carolinus

• Published in: Molecular ecology notes 2007-01, Vol.7 (1), p.141-143
• Main Authors: SEYOUM, SEIFU, DENISON, STEVEN H., TRINGALI, MICHAEL D.
• Format: Article
• Language: English
• Subjects: cross-species amplification; Florida pompano; microsatellites; Palometa; permit; Trachinotus; Trachinotus carolinus; Trachinotus falcatus; Trachinotus goodei
• ISSN: 1471-8278; 1471-8286
• DOI: 10.1111/j.1471-8286.2006.01556.x

Here we describe 13 polymorphic, dinucleotide microsatellite loci for Trachinotus carolinus (Florida pompano), isolated by using PIMA, a polymerase chain reaction‐based technique. The number of alleles at each locus ranged from 3 to 29 (mean = 11.4) in 45 specimens collected from bay and nearshore waters around St Petersburg, Florida. Levels of expected and observed heterozygosities ranged from 0.15 to 0.94 (mean = 0.69) and from 0.16 to 0.98 (mean = 0.70), respectively. No significant departures from Hardy–Weinberg equilibrium expectations were observed. In exact tests for genotypic disequilibrium, there was no evidence of linkage for any pair of loci. The ability of these markers to cross‐amplify in two congeneric Trachinotus species —T. falcatus (permit) and T. goodei (palometa) — was also assessed. The loci were well‐resolved, highly polymorphic, and independently segregating in these taxa, also suggesting a general utility for intraspecific studies, species identification, and investigation of interspecific hybridization.