set of EST-SSRs isolated from peach fruit transcriptome and their transportability across Prunus species

Twenty-one expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed in peach from a mesocarp cDNA library. Eighteen of them gave successful amplification in 22 peach genotypes and produced one to three alleles each with an average of 1.8 alleles per locus. The average value of...

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Bibliographic Details
Published in:Molecular ecology notes 2007-03, Vol.7 (2), p.307-310
Main Authors: VENDRAMIN, E, DETTORI, M.T, GIOVINAZZI, J, MICALI, S, QUARTA, R, VERDE, I
Format: Article
Language:English
Subjects:
EST
microsatellite
microsatellite repeats
peach
peaches
Prunus
Prunus dulcis
Prunus persica
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Summary:Twenty-one expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed in peach from a mesocarp cDNA library. Eighteen of them gave successful amplification in 22 peach genotypes and produced one to three alleles each with an average of 1.8 alleles per locus. The average value of expected and observed heterozygosities was 0.24 and 0.20, respectively. All the primers gave successful amplification in other six Prunus species (almond, apricot, sweet cherry, Japanese plum, European plum and Prunus ferganensis).
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2006.01590.x