Lactococcus lactis, causative agent of an endocarditis valvularis and parietalis thromboticans in the allis shad, Alosa alosa (L.)

• Published in: Journal of fish diseases 2018-08, Vol.41 (8), p.1207-1215
• Main Authors: Wünnemann, H, Eskens, U, Prenger‐Berninghoff, E, Ewers, C, Lierz, M
• Format: Article
• Language: English
• Subjects: 16S rRNA gene sequencing; Alosa alosa; Biochemical characteristics; Biochemistry; Breeding; Endocarditis; Fish; Freshwater fishes; histopathology; Infections; Infectious diseases; Ionization; Lactococcus lactis; Lasers; MALDI‐TOF MS; Mass spectrometry; Mass spectroscopy; Population decline; Reintroduction; reintroduction programme; rRNA 16S
• ISSN: 0140-7775; 1365-2761
• DOI: 10.1111/jfd.12813

Since the 1940s, the anadromous allis shad, Alosa alosa (L.), has suffered population declines throughout its distribution range in Europe. In context of EU‐LIFE projects for the reintroduction of the allis shad in the Rhine system, a comprehensive study was started in 2012 to investigate infectious diseases occurring in allis shad. In course of the study, 217 mature and young‐of‐the‐year allis shad originating from the wild population from the Gironde–Garonne–Dordogne system (GGD‐system) and the Rhine system as well as 38 allis shad from the breeding population were examined by use of bacteriological and histological methods. In 2012 and 2014, an endocarditis valvularis thromboticans caused by a coccoid bacterium was detected in 16% and 25% of mature allis shad originating from the GGD‐system. Results of microbiologic examinations, including biochemical characteristics, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and 16S rRNA gene sequence analysis, revealed Lactococcus lactis as causative agent of this infection. This is the first report of an endocarditis valvularis and parietalis thromboticans caused by Lactococcus lactis in fish. Possible sources of infection as well as the impact for the reintroduction programme are discussed.