In vitro and in vivo study of PCL/COLL wound dressing loaded with insulin-chitosan nanoparticles on cutaneous wound healing in rats model

• Published in: International journal of biological macromolecules 2018-10, Vol.117, p.601-609
• Main Authors: Ehterami, Arian, Salehi, Majid, Farzamfar, Saeed, Vaez, Ahmad, Samadian, Hadi, Sahrapeyma, Hamed, Mirzaii, Mehdi, Ghorbani, Sadegh, Goodarzi, Arash
• Format: Article
• Language: English
• Subjects: Chitosan; Collagen; Electrospinning; Insulin; Poly (ε-caprolactone); Skin wound-dressing
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2018.05.184

In the current study, insulin delivering chitosan nanoparticles were coated onto the electrospun poly (ε-caprolactone) (PCL)/Collagen (COLL) to produce a potential wound care material. Electrospun matrices were fabricated from PCL/COLL (1:1 (w/w)) solution. The insulin-loaded chitosan nanoparticles were produced by ionic gelation process and then attached onto the yarns. The dressings were investigated regarding their surface wettability, microstructure, the capacity to absorb water, water vapour permeability, mechanical properties, blood compatibility, microbial penetration, and cellular behavior. Full-thickness excisional wound model was used to assess the in vivo healing capacity of the dressings. Our data showed that after 14 days the wounds covered with PCL/COLL/Cs-Ins wound dressing could reach to nearly full wound closure compared with the sterile gauze which exhibited nearly 45% of wound size reduction. Our results suggest that fabricated scaffolds can be potentially applied in clinical practice for wound treatment.