Effects of seminal plasma and flash-freezing on DNA structure of stallion epididymal sperm exposed to different potentiators of DNA damage

The tolerance of sperm DNA structure to seminal plasma and freezing conditions has both clinical and basic biologic relevance. In this study, fresh (FS) or flash-frozen (FZ) stallion epididymal sperm were exposed (SP+) or unexposed (SP−) to seminal plasma. Sperm were then evaluated to monitor the de...

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Bibliographic Details
Published in:Theriogenology 2018-09, Vol.117, p.34-39
Main Authors: Serafini, R., Varner, D.D., Blanchard, T.L., Teague, S.R., LaCaze, K., Love, C.C.
Format: Article
Language:English
Subjects:
DNA structure
Flash-freezing
Potentiators
Seminal plasma
Sperm
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Summary:The tolerance of sperm DNA structure to seminal plasma and freezing conditions has both clinical and basic biologic relevance. In this study, fresh (FS) or flash-frozen (FZ) stallion epididymal sperm were exposed (SP+) or unexposed (SP−) to seminal plasma. Sperm were then evaluated to monitor the degree of change in DNA structure following challenge with chemical (dithiothreitol-DTT), oxidative (iron sulfate; FeSO4) or enzymatic (DNase I) potentiators of DNA damage. For sperm not treated with potentiators (controls), there was no effect of SP treatment (SP− vs. SP+) or freezing treatment (FS vs. FZ; non-significant) on measures of any DNA assays (i.e., 8-hydroxy, 2′deoxyguanosine [8OHdG], TUNEL, or sperm chromatin structure [SCSA] assays). Group FZ was more susceptible than Group FS to potentiators of DNA damage. Percent 8OHdG-positive sperm was higher in Group FZ/SP− treated with FeSO4 than all other groups (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2018.05.023