Callus Induction and in vitro Complete Plant Regeneration of Different Cultivars of Tobacco (Nicotiana tabacum L.) on Media of Different Hormonal Concentrations

• Published in: Biotechnology (Faisalābād, Pakistan) Pakistan), 2007-12, Vol.6 (4), p.561-566
• Main Authors: Ali, G, Hadi, F, Ali, Z, Tariq, M, Khan, MA
• Format: Article
• Language: English
• Subjects: Nicotiana tabacum
• ISSN: 1682-296X
• DOI: 10.3923/biotech.2007.561.566

In the present study, regeneration conditions for two-cultivars-of-tobacco (Nicotiana tabacum L.) were optimized. At different concentrations the effects of 1-naphthalene acetic acid (auxin)-and-6-benzylaminopurine (cytokinin)-on-callus-induction-and-subsequent-plant regeneration in K-399 and SPTG-172 using Murashige and Skoog (MS) media were studied. The seeds were germinated on simple MS media, while MS media with auxine and cytokinin was used for callus induction and regeneration of shoots. In tobacco, callus was successfully induced from leaf explants using a high ratio of auxin: Cytokinin. The over all callus induction frequency in SPTG-172 was greater than K-399 indicating a difference in the genetic background of two cultivars. Both cultivars also showed best response to different hormonal concentrations. The resultant calli were stimulated to form shoots by increasing the cytokinin concentration and decreasing auxin content of culture media. There were clear differences between the two cultivars in their ability to produce regenerated plants. The maximum number of calli were regenerated in SPTG-172 whereas the mean number of shoots produced per callus at all concentrations was higher in K-399 indicating that's shoot regeneration is markedly affected by the genotype. The roots developed well on hormones free MS media in both cultivars irrespectively of the varietal difference. The well-rooted plants were then transferred to green house for acclimatization-to-the in vitro environment.