Significance of treated agrowaste residue and autochthonous inoculates (Arbuscular mycorrhizal fungi and Bacillus cereus) on bacterial community structure and phytoextraction to remediate soils contaminated with heavy metals

• Published in: Chemosphere (Oxford) 2009-04, Vol.75 (3), p.327-334
• Main Authors: Azcón, Rosario, Medina, Almudena, Roldán, Antonio, Biró, Borbála, Vivas, Astrid
• Format: Article
• Language: English
• Subjects: Agriculture; Agrowaste residue; Animal, plant and microbial ecology; Applied ecology; Aspergillus; Aspergillus niger; Aspergillus niger - metabolism; Bacillus cereus; Bacillus cereus - metabolism; Bacteria - metabolism; Biodegradation, Environmental; Biodiversity; Biological and medical sciences; bioremediation; Ecotoxicology, biological effects of pollution; Fertilizers; Fundamental and applied biological sciences. Psychology; General aspects; heavy metals; Metals, Heavy - analysis; Microbial ecology; Mycorrhizae - metabolism; Mycorrhizal–bacterial inoculation; phosphorus fertilizers; Phytoextraction; phytoremediation; Plant Development; Plant Shoots - growth & development; polluted soils; pretreatment; Soil; soil amendments; soil bacteria; soil inoculation; Soil Microbiology; Soil Pollutants - analysis; species diversity; sugar beet pulp; Symbiosis; Trifolium - growth & development; Trifolium repens; Various environments (extraatmospheric space, air, water); vesicular arbuscular mycorrhizae
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2008.12.029

In this study, we analyzed the impact of treatments such as Aspergillus niger-treated sugar beet waste (SB), PO 4 3 - fertilization and autochthonous inoculants [arbuscular mycorrhizal (AM) fungi and Bacillus cereus], on the bacterial community structure in a soils contaminated with heavy metals as well as, the effectiveness on plant growth ( Trifolium repens). The inoculation with AM fungi in SB amended soil, increased plant growth similarly to PO 4 3 - addition, and both treatments matched in P acquisition but bacterial biodiversity estimated by denaturing gradient gel electrophoresis of amplified 16S rDNA sequences, was more stimulated by the presence of the AM fungus than by PO 4 3 - fertilization. The SB amendment plus AM inoculation increased the microbial diversity by 233% and also changed (by 215%) the structure of the bacterial community. The microbial inoculants and amendment used favoured plant growth and the phytoextraction process and concomitantly modified bacterial community in the rhizosphere; thus they can be used for remediation. Therefore, the understanding of such microbial ecological aspects is important for phytoremediation and the recovery of contaminated soils.