Efficient synthesis of gluco-oligosaccharides and alkyl-glucosides by transglycosylation activity of β-glucosidase from Sclerotinia sclerotiorum

An extracellular β-glucosidase (β-glu x) from Sclerotinia sclerotiorum was used as catalyst for the synthesis of gluco-oligosaccharides (GOSs) and alkyl-glucosides. The purified β-glu x was not regiospecific for β(1[rightward arrow]4) linkages in either hydrolysis or transglycosylation catalysed-rea...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2007-01, Vol.23 (1), p.145-149
Main Authors: Smaali, Issam, Maugard, Thierry, Limam, Ferid, Legoy, Marie-Dominique, Marzouki, Nejib
Format: Article
Language:English
Subjects:
Alkyl-glucosides
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
oligosaccharides
Sclerotinia sclerotiorum
Transglycosylation
β-glucosidase
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Summary:An extracellular β-glucosidase (β-glu x) from Sclerotinia sclerotiorum was used as catalyst for the synthesis of gluco-oligosaccharides (GOSs) and alkyl-glucosides. The purified β-glu x was not regiospecific for β(1[rightward arrow]4) linkages in either hydrolysis or transglycosylation catalysed-reactions. It efficiently synthesized GOSs from cellobiose, gentiobiose and methyl β-d-glucoside by transglycosylation. At optimal conditions, 119 mg/ml of GOSs (~~33%) were formed over 9 h from cellobiose as substrate. Alkyl-glucosides were also efficiently synthesized by transglycosylation of cellobiose in presence of different alcohols in biphasic media. However, their concentrations decreased as the size of the alcohol chain increased.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-006-9185-6