Multi-generation studies with Chironomus riparius – Effects of low tributyltin concentrations on life history parameters and genetic diversity

• Published in: Chemosphere (Oxford) 2007-05, Vol.67 (11), p.2192-2200
• Main Authors: Vogt, Christian, Nowak, Carsten, Diogo, Joao Barateiro, Oetken, Matthias, Schwenk, Klaus, Oehlmann, Jörg
• Format: Article
• Language: English
• Subjects: Adaptation; Adaptation, Physiological - drug effects; animal reproduction; Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Body Weight - drug effects; Chironomidae - drug effects; Chironomidae - genetics; Chironomidae - growth & development; Chironomus riparius; Drug Resistance; ecotoxicology; Ecotoxicology, biological effects of pollution; Freshwater; Fundamental and applied biological sciences. Psychology; General aspects; Genetic variability; genetic variation; Genetic Variation - drug effects; genotoxicity; insect genetics; insect growth; insect pests; insecticide resistance; insecticides; Larva - drug effects; life cycle (organisms); Microsatellite Repeats; Microsatellites; molecular sequence data; Multi-generation study; multi-generational study; Nonlinear Dynamics; population genetics; Reproduction - drug effects; Sex Ratio; stress tolerance; toxicity testing; Trialkyltin Compounds - analysis; Trialkyltin Compounds - toxicity; tributyltin oxide
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2006.12.025

While toxicological data are available for numerous chemicals from standard tests, little is known on effects of pollutants over several generations or regarding chronic effects of chemicals on genetic diversity. Within the experiments, effects of the model pollutant tributyltin (TBT) were investigated over eleven generations at a sublethal TBT concentration of 4.46 μg as Sn kg −1 sediment dw on life-cycle parameters and genetic variability of Chironomus riparius. Moreover, the adaptation potential towards TBT was determined. This experimental design enables the identification of TBT effects on life-cycle parameters and the determination of a potential extinction risk caused by chronic exposure. Furthermore, effects on the genetic structure can be determined, which are not predictable based solely on knowledge of the toxic mode of action of the chemical. Genetic variety was determined via microsatellite analysis, measuring individual length differences of highly variable satellite DNA fragments. For the identification of changes in tolerances towards the stressor, acute and chronic toxicity experiments were conducted. During the multi-generation study, significant effects on development and reproduction were determined. For some generations, the emergence was significantly ( p < 0.05) delayed under TBT exposure. Reproduction seems to be a sensitive parameter as well, whereby females laid significantly larger egg masses ( p < 0.05) in the latter generations. TBT did not affect the population growth rate nor the genetic variability, while clear deviations from the Hardy–Weinberg equilibrium appeared. The study also provides strong evidence for the acquirement of a higher tolerance towards the stressor in the TBT-exposed group.