The use of NTA and EDDS for enhanced phytoextraction of metals from a multiply contaminated soil by Brassica carinata

• Published in: Chemosphere (Oxford) 2007-08, Vol.68 (10), p.1920-1928
• Main Authors: Quartacci, Mike F., Irtelli, Barbara, Baker, Alan J.M., Navari-Izzo, Flavia
• Format: Article
• Language: English
• Subjects: (S,S)-ethylenediamine disuccinic acid; Applied sciences; arsenic; bioaccumulation; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; Brassica - growth & development; Brassica - metabolism; Brassica carinata; cadmium; Chelants; chelating agents; copper; Decontamination. Miscellaneous; Desorption; Earth sciences; Earth, ocean, space; Engineering and environment geology. Geothermics; Environment and pollution; Ethylenediamines - chemistry; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; lead; metals; Metals, Heavy - chemistry; Metals, Heavy - metabolism; Miscellaneous; nitrilotriacetic acid; Nitrilotriacetic Acid - chemistry; Phytoextraction; phytoremediation; Plant Roots - growth & development; polluted soils; Pollution; Pollution, environment geology; shoots; Soil and sediments pollution; Soil Pollutants - chemistry; Soil Pollutants - metabolism; soil pollution; soil treatment; Succinates - chemistry; zinc
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2007.02.058

The potential of nine different species to grow in the presence of metals (As, Cd, Cu, Pb and Zn) and to accumulate them in the shoots was assessed for each metal separately by germination and root length tests, and successively by hydroponic experiments. Of the nine species tested, Brassica carinata was the species that accumulated the highest amounts of metals in shoots without suffering a significant biomass reduction. To further evaluate the potential of B. carinata for chelant-enhanced phytoextraction of a natural, multiply metal-polluted soil (As, Cd, Cu, Pb and Zn), both hydroponic and pot experiments were carried out with nitrilotriacetic acid (NTA) or ( S, S)-ethylenediamine disuccinic acid (EDDS) as complexing agents. The hydroponic study with solutions containing the five metals together showed that accumulation of Cd, Cu, Pb and Zn in shoots was higher following EDDS addition compared to NTA. EDDS was more effective than NTA in desorbing Cu, Pb and Zn from the soil, whereas As and Cd were poorly extracted. B. carinata plants were grown for 4 weeks in the multiply metal-contaminated soil and then the soil was amended with 5 mmol kg −1 NTA or EDDS. All plants were harvested 1 week after amendment. In comparison to NTA, EDDS was more effective in enhancing the concentrations of Cu, Pb and Zn in B. carinata shoots (2- to 4-fold increase compared to the control). One week after chelant addition, the DTPA-extractable metal concentrations in the polluted soil were lower in the EDDS treatment in comparison with the NTA amendment. Even though B. carinata showed a reduced growth and a relatively low metal uptake, it demonstrated the ability to survive and tolerate the presence of more metals simultaneously.