Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally po...

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Bibliographic Details
Published in:Optics letters 2018-06, Vol.43 (11), p.2587-2590
Main Authors: Nygate, Yoav N, Singh, Gyanendra, Barnea, Itay, Shaked, Natan T
Format: Article
Language:English
Subjects:
Acridine Orange - pharmacology
Beads
Colonic Neoplasms - drug therapy
Colonic Neoplasms - pathology
Equipment Design
Fluorescence
Fluorescent Dyes - pharmacology
Fourier Analysis
Holography
Holography - methods
Humans
Imaging
Interferometry
Microscopy
Microscopy, Fluorescence - methods
Microspheres
Multimodal Imaging
Multiplexing
Plasma
Tumor Cells, Cultured
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Summary:We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope. In contrast to previous approaches, the presented technique allows conventional fluorescence imaging, rather than interferometric off-axis fluorescence imaging. We demonstrate the presented technique for imaging fluorescent beads and live biological cells.
ISSN:0146-9592
1539-4794
DOI:10.1364/ol.43.002587