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Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally po...
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| Published in: | Optics letters 2018-06, Vol.43 (11), p.2587-2590 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c423t-4280760c63bf8abc489178ac99bc1e358f067236b259b4bd6a16db9cec667eb83 |
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| cites | cdi_FETCH-LOGICAL-c423t-4280760c63bf8abc489178ac99bc1e358f067236b259b4bd6a16db9cec667eb83 |
| container_end_page | 2590 |
| container_issue | 11 |
| container_start_page | 2587 |
| container_title | Optics letters |
| container_volume | 43 |
| creator | Nygate, Yoav N Singh, Gyanendra Barnea, Itay Shaked, Natan T |
| description | We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope. In contrast to previous approaches, the presented technique allows conventional fluorescence imaging, rather than interferometric off-axis fluorescence imaging. We demonstrate the presented technique for imaging fluorescent beads and live biological cells. |
| doi_str_mv | 10.1364/ol.43.002587 |
| format | article |
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| fulltext | fulltext |
| identifier | ISSN: 0146-9592 |
| ispartof | Optics letters, 2018-06, Vol.43 (11), p.2587-2590 |
| issn | 0146-9592 1539-4794 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2049555017 |
| source | OSA_美国光学学会数据库1 |
| subjects | Acridine Orange - pharmacology Beads Colonic Neoplasms - drug therapy Colonic Neoplasms - pathology Equipment Design Fluorescence Fluorescent Dyes - pharmacology Fourier Analysis Holography Holography - methods Humans Imaging Interferometry Microscopy Microscopy, Fluorescence - methods Microspheres Multimodal Imaging Multiplexing Plasma Tumor Cells, Cultured |
| title | Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells |
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