Chitosan/hyaluronan/edaravone membranes for anti-inflammatory wound dressing: In vitro and in vivo evaluation studies

• Published in: Materials Science & Engineering C 2018-09, Vol.90, p.227-235
• Main Authors: Tamer, Tamer M., Valachová, Katarína, Hassan, Mohamed A., Omer, Ahmed M., El-Shafeey, Muhammad, Mohy Eldin, Mohamed S., Šoltés, Ladislav
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Anti-inflammatory drugs; Antipyrine - analogs & derivatives; Antipyrine - chemistry; Bandages; Carbohydrates; Chitosan; Chitosan - chemistry; Comparative analysis; Comparative studies; Cotton; Decoloring; Decolorization; Edaravone; Female; Free radicals; Gauze; Hyaluronan; Hyaluronic acid; Hyaluronic Acid - chemistry; In vivo methods and tests; Inflammation; Materials science; Membranes; Rats; Scavenging; Simulation; Skin; Spectroscopy, Fourier Transform Infrared; Surgery; Thermal properties; Thermodynamic properties; Thermogravimetry; Wettability; Wound dressing membrane; Wound healing; Wound Healing - drug effects
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2018.04.053

A novel wound healing material composed of chitosan (Ch) and hyaluronan (HA) boosted with edaravone (Ed) as an anti-inflammatory drug was developed. The fabricated membranes were verified using FT-IR, and the thermal properties were estimated employing TGA instrument. Moreover, Physical characterizations of the prepared membranes demonstrated a decrease in the membrane wettability, whereas an increase in membrane roughness was monitored due to the effect of edaravone supplementation. A comparative study of free-radical scavenging activity of edaravone itself was carried out by two in vitro approaches: uninhibited/inhibited hyaluronan degradation and decolorization of ABTS methods in normal and simulated inflammation condition (acidic condition). Accordingly, the scavenging activity of edaravone was significantly diminished to OH and peroxy−/alkoxy-type radicals in acidic conditions in compared to the neutral reactions. The biochemical studies evidenced the haemocompatibility of the examined membranes. The consequence of membranes composed of Ch/HA/Ed on the wound healing of the rat's skin was studied, and the macroscopic and microscopic investigations revealed remarkable healing at 21st day post-surgery compared with injuries treated with cotton gauze as a negative control in addition to Ch/HA membrane without edaravone. For these reasons, the Ch/HA/Ed membrane could be implemented as wound dressing material. [Display omitted] •Polyelectrolyte membrane of chitosan and hyaluronan in addition of edaravone was prepared and characterized.•In vitro examinations of membranes such as haemocompatibility and antioxidant activity were evaluated.•In vivo studies of membranes demonstrate significant improvements of wound healing in the presence of edaravone.