Asparagus densiflorus in a vertical subsurface flow phytoreactor for treatment of real textile effluent: A lab to land approach for in situ soil remediation

• Published in: Ecotoxicology and environmental safety 2018-10, Vol.161, p.70-77
• Main Authors: Watharkar, Anuprita D., Kadam, Suhas K., Khandare, Rahul V., Kolekar, Parag D., Jeon, Byong-Hun, Jadhav, Jyoti P., Govindwar, Sanjay P.
• Format: Article
• Language: English
• Subjects: A. densiflorus; Ammonium Compounds - metabolism; Aniline Compounds - metabolism; Asparagus Plant - enzymology; Azo Compounds - metabolism; Biodegradation, Environmental; Coloring Agents - metabolism; Coloring Agents - toxicity; Crops, Agricultural - drug effects; Environmental Restoration and Remediation - methods; Gas Chromatography-Mass Spectrometry; Hep G2 Cells; Humans; Industrial Waste; Laccase; Nitriles - metabolism; Oxidoreductases - metabolism; Peroxidases; Phytoremediation; Plant Roots - enzymology; Rubin GFL; Soil - chemistry; Soil Pollutants - metabolism; Textile effluent; Textile Industry; Textiles; Toxicity; Vertical subsurface flow phytoreactor; Waste Water - chemistry; Water Pollutants, Chemical - metabolism
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2018.05.078

This study explores the potential of Asparagus densiflorus to treat disperse Rubin GFL (RGFL) dye and a real textile effluent in constructed vertical subsurface flow (VSbF) phytoreactor; its field cultivation for soil remediation offers a real green and economic way of environmental management. A. densiflorus decolorized RGFL (40 gm L−1) up to 91% within 48 h. VSbF phytoreactor successfully reduced American dye manufacture institute (ADMI), BOD, COD, Total Dissolved Solids (TDS) and Total Suspended Solids (TSS) of real textile effluent by 65%, 61%, 66%, 48% and 66%, respectively within 6 d. Oxidoreductive enzymes such as laccase (138%), lignin peroxidase (129%), riboflavin reductase (111%) were significantly expressed during RGFL degradation in A. densiflorus roots, while effluent transformation caused noteworthy induction of enzymes like, tyrosinase (205%), laccase (178%), veratryl oxidase (52%). Based on enzyme activities, UV–vis spectroscopy, FTIR and GC-MS results; RGFL was proposed to be transformed to 4-amino-3- methylphenyl (hydroxy) oxoammonium and N, N-diethyl aniline. Anatomical study of the advanced root tissue of A. densiflorus exhibited the progressive dye accumulation and removal during phytoremediation. HepG2 cell line and phytotoxicity study demonstrated reduced toxicity of biotransformed RGFL and treated effluent by A. densiflorus, respectively. On field remediation study revealed a noteworthy removal (67%) from polluted soil within 30 d. [Display omitted] •Asparagus densiflorus showed potential to transform disperse dye Rubin GFL.•Vertical subsurface flow phytoreactor efficiently decolorized real textile effluent.•Toxicity study confirmed the reduced toxicity of biotransformed dye and effluent.•In situ soil remediation studies revealed a noteworthy removal of soil ADMI.•Lab to land transfer of phytoremediation technology was successfully achieved.