Mutagenic and recombinagenic activity of airborne particulates, PM10 and TSP, organic extracts in the Drosophila wing-spot test

The genotoxicity associated with air pollution in the city of Canoas, Rio Grande do Sul (Brazil), was assessed in November (spring) and January (summer). We applied the somatic mutation and recombination test (SMART) in Drosophila melanogaster in its standard version with normal bioactivation (ST) a...

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Bibliographic Details
Published in:Environmental pollution (1987) 2008, Vol.151 (1), p.47-52
Main Authors: Dihl, Rafael Rodrigues, da Silva, Carla Grazielli Azevedo, Amaral, Viviane Souza do, Reguly, Maria Luiza, de Andrade, Heloísa Helena Rodrigues
Format: Article
Language:English
Subjects:
Air Pollutants - toxicity
air pollution
Air Pollution - adverse effects
Air Pollution - analysis
Airborne particulates
Animal, plant and microbial ecology
Animals
Applied ecology
bioassays
Biological and medical sciences
Brazil
chromosome aberrations
cytochrome P-450
Drosophila melanogaster
Drosophila melanogaster - drug effects
Drosophila melanogaster - genetics
Ecotoxicology, biological effects of pollution
extracts
Fundamental and applied biological sciences. Psychology
General aspects
Genes, Insect
genetic recombination
Genotoxicity
mitosis
Mitotic recombination
Mutagenicity Tests - methods
mutants
mutation
organic compounds
Particulate Matter
particulates
Recombination, Genetic
seasonal variation
Seasons
SMART
somatic mutation and recombination test
strain differences
strains
Terrestrial environment, soil, air
Wings, Animal - anatomy & histology
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Summary:The genotoxicity associated with air pollution in the city of Canoas, Rio Grande do Sul (Brazil), was assessed in November (spring) and January (summer). We applied the somatic mutation and recombination test (SMART) in Drosophila melanogaster in its standard version with normal bioactivation (ST) and in its variant with increased cytochrome P450-dependent biotransformation capacity (HB). The data indicated the genotoxicity of TSP and PM10 collected in November, in both ST and HB crosses. The genotoxic activity of the PM10 material in the spring sample was exclusively associated with the induction of mitotic recombination, whereas the TSP genetic toxicity was due to both recombinational as well as point and/or chromosomal mutation events. Considering PM10 collected in January, a positive response—100% (17.10 m 3/ml) concentration—was observed in the HB cross, which was not detected in the ST cross. Drosophila Wing-Spot Test can be used for detection of airborne particulates mutagenesis.
ISSN:0269-7491
1873-6424
DOI:10.1016/j.envpol.2007.03.008