Construction of an infectious full-length cDNA clone of Chrysanthemum virus B

An infectious full-length cDNA clone of Chrysanthemum virus B (CVB, genus Carlavirus ), was constructed. Four cDNA fragments covering the whole genome of CVB-S were cloned between the Cauliflower mosaic virus 35S promoter and the nopaline synthase (NOS) terminator. Chrysanthemum and garland chrysant...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2008-12, Vol.74 (6), p.434-437
Main Authors: Ohkawa, A.(Utsunomiya Univ. (Japan). Coll. of Agriculture), Ishikawa Suehiro, N, Okuda, S, Natsuaki, T
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
CARLAVIRUS
CARLAVIRUSES
Cauliflower mosaic virus
CHRYSANTHEME
Chrysanthemum
Chrysanthemum virus b
CHRYSANTHEMUMS
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
CLONE
CLONES
Cloning
CRISANTEMOS
Deoxyribonucleic acid
DNA
FONCTION PHYSIOLOGIQUE
FUNCION FISIOLOGICA
Genomics
INOCULACION
INOCULATION
Life Sciences
Microbiology
MOLECULAR CLONING
PHYSIOLOGICAL FUNCTIONS
Plant Pathology
Viral and Viroid Diseases
Viruses
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Summary:An infectious full-length cDNA clone of Chrysanthemum virus B (CVB, genus Carlavirus ), was constructed. Four cDNA fragments covering the whole genome of CVB-S were cloned between the Cauliflower mosaic virus 35S promoter and the nopaline synthase (NOS) terminator. Chrysanthemum and garland chrysanthemum were inoculated with the constructed plasmid, named pCVB, using a gene gun system. As is the case in wild-type, CVB-infected plants, no visible symptoms were observed on plants inoculated with pCVB; however, western blotting and electron microscopy indicated the presence of the progeny virus of pCVB. pCVB could be a useful tool for analyzing the functions of carlaviral proteins.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-008-0120-6