New Meredithiella species from mycangia of Corthylus ambrosia beetles suggest genus-level coadaptation but not species-level coevolution

• Published in: Mycologia 2018-01, Vol.110 (1), p.63-78
• Main Authors: Mayers, Chase G., Bateman, Craig C., Harrington, Thomas C.
• Format: Article
• Language: English
• Subjects: 2 new taxa; Adaptation, Biological; Ambrosia - parasitology; Ambrosia symbiosis; Americas; Animals; Ascomycota - classification; Ascomycota - genetics; Ascomycota - growth & development; Ascomycota - isolation & purification; Cluster Analysis; DNA, Fungal - chemistry; DNA, Fungal - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Ecology; Evolution, Molecular; Microascales; Microscopy; mutualism; Peptide Elongation Factor 1 - genetics; Phylogeny; RNA, Ribosomal, 18S - genetics; RNA, Ribosomal, 28S - genetics; Scolytinae; Sequence Analysis, DNA; Symbiosis; Weevils - microbiology; X-Ray Microtomography
• ISSN: 0027-5514; 1557-2536
• DOI: 10.1080/00275514.2017.1400353

Meredithiella norrisii (Microascales, Ceratocystidaceae) is an ambrosia fungus carried in mycangia of the North American ambrosia beetle, Corthylus punctatissimus. Reports on the identity of the fungal symbionts of other species of Corthylus have been inconsistent. This study tested the hypothesis that Meredithiella spp. are the primary symbionts of Corthylus spp. Cultures and/or internal transcribed spacer (ITS) rDNA barcode sequences of Meredithiella spp. were obtained consistently from beetles and galleries of nine Corthylus spp. The ITS sequences of three putative species of Meredithiella were associated with C. consimilis and C. flagellifer in Mexico and C. calamarius in Costa Rica. The symbiont of C. columbianus in the USA was identified as M. norrisii. Two new Meredithiella spp. are described: M. fracta from C. papulans in Florida and Honduras, and M. guianensis associated with C. crassus and two unidentified Corthylus spp. in French Guyana. The Meredithiella spp. propagate in the mycangia of adult females by thallic-arthric growth, and the ambrosia growth in larval cradles comprises bead-like hyphal swellings or conidiophores, with or without terminal aleurioconidia. Bayesian phylogenetic analysis of a combined 18S and 28S nuc rDNA and translation elongation factor 1-α (TEF1-α) data set demonstrated that Meredithiella is a distinct monophyletic clade within the Ceratocystidaceae, but its phylogenetic placement with regard to the other ambrosial genera in the family remains ambiguous. The mycangia of C. punctatissimus and C. papulans are also compared using light microscopy and micro-computed tomography (micro-CT) imaging, revealing that they differ in both size and shape, but these differences may not correlate with different lineages of Meredithiella.