The changes of bacterial communities and antibiotic resistance genes in microbial fuel cells during long-term oxytetracycline processing

• Published in: Water research (Oxford) 2018-10, Vol.142, p.105-114
• Main Authors: Yan, Weifu, Guo, Yunyan, Xiao, Yong, Wang, Shuhua, Ding, Rui, Jiang, Jiaqi, Gang, Haiyin, Wang, Han, Yang, Jun, Zhao, Feng
• Format: Article
• Language: English
• Subjects: Antibiotic degradation; Antibiotic resistance gene; Bacteria - genetics; Bacteria - metabolism; Bioelectric Energy Sources - microbiology; Biofilms; Drug Resistance, Microbial - genetics; Electrodes; Electron; Eubacterium - genetics; Eubacterium - metabolism; Genes, Bacterial; High-Throughput Nucleotide Sequencing; Microbial Consortia - genetics; Microbial fuel cell; Oxytetracycline; Oxytetracycline - metabolism; Transposases - genetics; Water Pollutants, Chemical - metabolism
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2018.05.047

Microbial fuel cell (MFC) is regarded as a promising alternative for enhancing the removal of antibiotic pollutants. In this study, oxytetracycline served as an electron donor in the anode chamber of MFCs, and after continuous operation for 330 days, the efficiency of removal of 10 mg/L oxytetracycline in MFCs increased to 99.00% in 78 h, whereas removal efficiency of only 58.26% was achieved in microbial controls. Compared to microbial controls, higher ATP concentration and persistent electrical stimulation mainly contributed to bioelectrochemical reactions more rapidly to enhance oxytetracycline removal in MFCs. In addition, the analysis of bacterial communities revealed that Eubacterium spp.—as the main functional bacterial genus responsible for oxytetracycline biodegradation—flourished starting from merely 0.00%–91.69% ± 0.27% (mean ± SD) in MFCs. High-throughput quantitative PCR showed that the normalized copy numbers of total antibiotic resistance genes (ARGs) and mobile genetic elements in MFCs were 1.7364 and 0.0065 copies/cell respectively, which were markedly lower than those in the microbial controls. Furthermore, there was no significant correlation between oxytetracycline concentration in the influent and abundance of ARGs in effluent from MFCs. Nevertheless, Tp614, a transposase gene, was found to be enriched in both MFCs and microbial reactors, suggesting that it may be a common challenge for different biological processes for wastewater treatment. This study therefore showed a lower probability of upregulation and transmission of ARGs in MFCs when compared to a traditional anaerobic microbial treatment. [Display omitted] •MFC enhanced degradation of 10 mg/L oxytetracycline.•Eubacterium spp. as functional genus biodegrading oxytetracycline flourished greatly.•ARGs in MFCs biofilm could have a lower possibility to horizontal transfer.•Tp614 was enriched in all effluents of biological reactors.