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Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor
Enzymatic acidolysis of refined, bleached and deodorized (RBD) palm olein with caprylic acid was carried out in a continuous packed bed bioreactor to produce structured lipid (SL) that can confer metabolic benefits when consumed. Lipozyme ® IM 60 from Rhizomucor miehei, a 1,3-specific lipase, was us...
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Published in: | Food chemistry 2005-09, Vol.92 (3), p.527-533 |
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creator | Lai, O.M. Low, C.T. Akoh, C.C. |
description | Enzymatic acidolysis of refined, bleached and deodorized (RBD) palm olein with caprylic acid was carried out in a continuous packed bed bioreactor to produce structured lipid (SL) that can confer metabolic benefits when consumed. Lipozyme
® IM 60 from
Rhizomucor miehei, a 1,3-specific lipase, was used as the biocatalyst in this study. After 24 h of reaction, 30.5% of the total fatty acid content of the modified oil was found to be caprylic acid, indicating its incorporation into the palm olein. The triacylglycerols (TAGs) of palm olein after acidolysis were separated and were characterized by seven clusters of TAG species with equivalent carbon number (ECN), C28, C30, C32, C34, C36, C38 and C40. Caprylic–oleic–caprylic TAGs were predicted in cluster C32, which recorded the highest amount, with 35.3% of the total TAG. Fatty acid composition at the
sn-2 position was determined, by pancreatic lipolysis, as C8:0, 9.2%; C12:0, 2.3%; C14:0, 1.8%; C16:0, 21.3%; C18:0, 4.7%; C18:1, 60.7%. Iodine value (IV), slip melting point (SMP) and differential scanning calorimetric (DSC) analyses of SL were also performed. In IV analysis, SL recorded a drop of value from 60.4 to 48.2 while SMP was reduced from 13 to 4.2 °C, in comparison to RBD palm olein. DSC analysis of SL gave a melting profile with two low melting peaks of −15.97 and −11.78 °C and onset temperatures of −18.43 and −14.03 °C, respectively. |
doi_str_mv | 10.1016/j.foodchem.2004.08.028 |
format | article |
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® IM 60 from
Rhizomucor miehei, a 1,3-specific lipase, was used as the biocatalyst in this study. After 24 h of reaction, 30.5% of the total fatty acid content of the modified oil was found to be caprylic acid, indicating its incorporation into the palm olein. The triacylglycerols (TAGs) of palm olein after acidolysis were separated and were characterized by seven clusters of TAG species with equivalent carbon number (ECN), C28, C30, C32, C34, C36, C38 and C40. Caprylic–oleic–caprylic TAGs were predicted in cluster C32, which recorded the highest amount, with 35.3% of the total TAG. Fatty acid composition at the
sn-2 position was determined, by pancreatic lipolysis, as C8:0, 9.2%; C12:0, 2.3%; C14:0, 1.8%; C16:0, 21.3%; C18:0, 4.7%; C18:1, 60.7%. Iodine value (IV), slip melting point (SMP) and differential scanning calorimetric (DSC) analyses of SL were also performed. In IV analysis, SL recorded a drop of value from 60.4 to 48.2 while SMP was reduced from 13 to 4.2 °C, in comparison to RBD palm olein. DSC analysis of SL gave a melting profile with two low melting peaks of −15.97 and −11.78 °C and onset temperatures of −18.43 and −14.03 °C, respectively.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2004.08.028</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Acidolysis ; Biological and medical sciences ; Bioreactors ; Biotechnology ; Caprylic acid ; continuous systems ; Enzymatic synthesis ; enzymatic treatment ; Fat industries ; fatty acid composition ; food analysis ; Food industries ; food quality ; Fundamental and applied biological sciences. Psychology ; iodine value ; Lipozyme ® IM 60 ; melting point ; Methods. Procedures. Technologies ; octanoic acid ; olein ; Packed bed bioreactor ; palm oils ; RBD palm olein ; Rhizomucor miehei ; Structured lipids ; triacylglycerol lipase ; triacylglycerols ; Various methods and equipments ; yields</subject><ispartof>Food chemistry, 2005-09, Vol.92 (3), p.527-533</ispartof><rights>2004 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-e3ebc6d60a82ccb41a1c5f092208702cf5ef0c0d1a2f3d693a8395f7a97bed8c3</citedby><cites>FETCH-LOGICAL-c511t-e3ebc6d60a82ccb41a1c5f092208702cf5ef0c0d1a2f3d693a8395f7a97bed8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16695914$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Lai, O.M.</creatorcontrib><creatorcontrib>Low, C.T.</creatorcontrib><creatorcontrib>Akoh, C.C.</creatorcontrib><title>Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor</title><title>Food chemistry</title><description>Enzymatic acidolysis of refined, bleached and deodorized (RBD) palm olein with caprylic acid was carried out in a continuous packed bed bioreactor to produce structured lipid (SL) that can confer metabolic benefits when consumed. Lipozyme
® IM 60 from
Rhizomucor miehei, a 1,3-specific lipase, was used as the biocatalyst in this study. After 24 h of reaction, 30.5% of the total fatty acid content of the modified oil was found to be caprylic acid, indicating its incorporation into the palm olein. The triacylglycerols (TAGs) of palm olein after acidolysis were separated and were characterized by seven clusters of TAG species with equivalent carbon number (ECN), C28, C30, C32, C34, C36, C38 and C40. Caprylic–oleic–caprylic TAGs were predicted in cluster C32, which recorded the highest amount, with 35.3% of the total TAG. Fatty acid composition at the
sn-2 position was determined, by pancreatic lipolysis, as C8:0, 9.2%; C12:0, 2.3%; C14:0, 1.8%; C16:0, 21.3%; C18:0, 4.7%; C18:1, 60.7%. Iodine value (IV), slip melting point (SMP) and differential scanning calorimetric (DSC) analyses of SL were also performed. In IV analysis, SL recorded a drop of value from 60.4 to 48.2 while SMP was reduced from 13 to 4.2 °C, in comparison to RBD palm olein. DSC analysis of SL gave a melting profile with two low melting peaks of −15.97 and −11.78 °C and onset temperatures of −18.43 and −14.03 °C, respectively.</description><subject>Acidolysis</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Caprylic acid</subject><subject>continuous systems</subject><subject>Enzymatic synthesis</subject><subject>enzymatic treatment</subject><subject>Fat industries</subject><subject>fatty acid composition</subject><subject>food analysis</subject><subject>Food industries</subject><subject>food quality</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>iodine value</subject><subject>Lipozyme ® IM 60</subject><subject>melting point</subject><subject>Methods. Procedures. Technologies</subject><subject>octanoic acid</subject><subject>olein</subject><subject>Packed bed bioreactor</subject><subject>palm oils</subject><subject>RBD palm olein</subject><subject>Rhizomucor miehei</subject><subject>Structured lipids</subject><subject>triacylglycerol lipase</subject><subject>triacylglycerols</subject><subject>Various methods and equipments</subject><subject>yields</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkE2P1DAMhisEEsPCX4Be4NbiJP1Ib6AVHyuNxAH2HHkch83QNiXpIA2_npRZxHEPViTreW3nKYqXAmoBont7rF0Ilu54qiVAU4OuQepHxU7oXlU99PJxsQMFutKi6Z4Wz1I6AoAEoXfFtPcLJq4IVxzPv9mWSN6G8Zx8KoMrFxynMozs5xJnWxIu8Tx6-kuVW7OkMK9-PoVTKg88012VCEfOQfqRpx228iEy0hri8-KJwzHxi_v3qrj9-OHb9edq_-XTzfX7fUWtEGvFig_U2Q5QS6JDI1BQ62CQEnQPklzLDgisQOmU7QaFWg2t63Ho8z5N6qp4c5m7xPDzxGk1k0_E44gz50ONhBbappMZ7C4gxZBSZGeW6CeMZyPAbHbN0fyzaza7BrTJdnPw9f0G3P7rIs7k0_901w3tIJrMvbpwDoPB7zEzt1-zegUCWqFBZeLdheAs5JfnaBL5LJKtj0yrscE_dMwfsESemg</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Lai, O.M.</creator><creator>Low, C.T.</creator><creator>Akoh, C.C.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050901</creationdate><title>Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor</title><author>Lai, O.M. ; Low, C.T. ; Akoh, C.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-e3ebc6d60a82ccb41a1c5f092208702cf5ef0c0d1a2f3d693a8395f7a97bed8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acidolysis</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Caprylic acid</topic><topic>continuous systems</topic><topic>Enzymatic synthesis</topic><topic>enzymatic treatment</topic><topic>Fat industries</topic><topic>fatty acid composition</topic><topic>food analysis</topic><topic>Food industries</topic><topic>food quality</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>iodine value</topic><topic>Lipozyme ® IM 60</topic><topic>melting point</topic><topic>Methods. Procedures. Technologies</topic><topic>octanoic acid</topic><topic>olein</topic><topic>Packed bed bioreactor</topic><topic>palm oils</topic><topic>RBD palm olein</topic><topic>Rhizomucor miehei</topic><topic>Structured lipids</topic><topic>triacylglycerol lipase</topic><topic>triacylglycerols</topic><topic>Various methods and equipments</topic><topic>yields</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lai, O.M.</creatorcontrib><creatorcontrib>Low, C.T.</creatorcontrib><creatorcontrib>Akoh, C.C.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lai, O.M.</au><au>Low, C.T.</au><au>Akoh, C.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor</atitle><jtitle>Food chemistry</jtitle><date>2005-09-01</date><risdate>2005</risdate><volume>92</volume><issue>3</issue><spage>527</spage><epage>533</epage><pages>527-533</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>Enzymatic acidolysis of refined, bleached and deodorized (RBD) palm olein with caprylic acid was carried out in a continuous packed bed bioreactor to produce structured lipid (SL) that can confer metabolic benefits when consumed. Lipozyme
® IM 60 from
Rhizomucor miehei, a 1,3-specific lipase, was used as the biocatalyst in this study. After 24 h of reaction, 30.5% of the total fatty acid content of the modified oil was found to be caprylic acid, indicating its incorporation into the palm olein. The triacylglycerols (TAGs) of palm olein after acidolysis were separated and were characterized by seven clusters of TAG species with equivalent carbon number (ECN), C28, C30, C32, C34, C36, C38 and C40. Caprylic–oleic–caprylic TAGs were predicted in cluster C32, which recorded the highest amount, with 35.3% of the total TAG. Fatty acid composition at the
sn-2 position was determined, by pancreatic lipolysis, as C8:0, 9.2%; C12:0, 2.3%; C14:0, 1.8%; C16:0, 21.3%; C18:0, 4.7%; C18:1, 60.7%. Iodine value (IV), slip melting point (SMP) and differential scanning calorimetric (DSC) analyses of SL were also performed. In IV analysis, SL recorded a drop of value from 60.4 to 48.2 while SMP was reduced from 13 to 4.2 °C, in comparison to RBD palm olein. DSC analysis of SL gave a melting profile with two low melting peaks of −15.97 and −11.78 °C and onset temperatures of −18.43 and −14.03 °C, respectively.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.foodchem.2004.08.028</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Acidolysis Biological and medical sciences Bioreactors Biotechnology Caprylic acid continuous systems Enzymatic synthesis enzymatic treatment Fat industries fatty acid composition food analysis Food industries food quality Fundamental and applied biological sciences. Psychology iodine value Lipozyme ® IM 60 melting point Methods. Procedures. Technologies octanoic acid olein Packed bed bioreactor palm oils RBD palm olein Rhizomucor miehei Structured lipids triacylglycerol lipase triacylglycerols Various methods and equipments yields |
title | Lipase-catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor |
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