microRNA downregulation in plasmacytoid dendritic cells in interferon-positive systemic lupus erythematosus and antiphospholipid syndrome

Abstract Objective To investigate miRNA expression in relation to transcriptomic changes in plasmacytoid dendritic cells (pDCs) in SLE and APS. pDCs are major producers of IFNα in SLE and APS, and miRNAs are emerging as regulators of pDC activation. Methods miRNA and mRNA expression were measured by...

Full description

Saved in:
Bibliographic Details
Published in:Rheumatology (Oxford, England) England), 2018-09, Vol.57 (9), p.1669-1674
Main Authors: van den Hoogen, Lucas L, Rossato, Marzia, Lopes, Ana P, Pandit, Aridaman, Bekker, Cornelis P J, Fritsch-Stork, Ruth D E, van Roon, Joel A G, Radstake, Timothy R D J
Format: Article
Language:English
Subjects:
Adult
Antiphospholipid syndrome
Antiphospholipid Syndrome - genetics
Antiphospholipid Syndrome - metabolism
Apoptosis
Autoimmune diseases
Dendritic cells
Dendritic Cells - metabolism
Dendritic Cells - pathology
Down-Regulation
Female
Gene expression
Gene Expression Regulation
Humans
Interferon
Lupus
Lupus Erythematosus, Systemic - genetics
Lupus Erythematosus, Systemic - metabolism
Lupus Erythematosus, Systemic - pathology
Male
MicroRNAs
MicroRNAs - biosynthesis
MicroRNAs - genetics
miRNA
Molecular modelling
RNA, Messenger - genetics
Systemic lupus erythematosus
TLR7 protein
Toll-Like Receptor 7 - biosynthesis
Toll-Like Receptor 7 - genetics
Toll-like receptors
α-Interferon
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Objective To investigate miRNA expression in relation to transcriptomic changes in plasmacytoid dendritic cells (pDCs) in SLE and APS. pDCs are major producers of IFNα in SLE and APS, and miRNAs are emerging as regulators of pDC activation. Methods miRNA and mRNA expression were measured by OpenArray and RNA-sequencing in pDCs of SLE, SLE + APS (APS secondary to SLE) and primary APS (PAPS) patients. The miRNA profile of patients was compared with the miRNA pattern of TLR7-activated pDCs. Results Among 131 miRNAs detected in pDCs, 35, 17 and 21 had a significantly lower level of expression in SLE, SLE + APS and PAPS patients, respectively, as compared with healthy controls (HC). Notably, the miRNA profile did not significantly differ between SLE and APS, but was driven by the presence or absence of an IFN signature. TLR7 stimulation induced a general downregulation of miRNAs, similar to the pattern observed in SLE and APS patients. miR-361-5p, miR-128-3p and miR-181a-2-3p expression was lower in patients with a high IFN signature (false discovery rate
ISSN:1462-0324
1462-0332
DOI:10.1093/rheumatology/key159