Ketohexokinase knockout mice, a model for essential fructosuria, exhibit altered fructose metabolism and are protected from diet-induced metabolic defects

• Published in: American journal of physiology: endocrinology and metabolism 2018-09, Vol.315 (3), p.E386-E393
• Main Authors: Miller, Corin O, Yang, Xiaodong, Lu, Ku, Cao, Jin, Herath, Kithsiri, Rosahl, Thomas W, Askew, Roger, Pavlovic, Guillaume, Zhou, Gaochao, Li, Cai, Akiyama, Taro E
• Format: Article
• Language: English
• Subjects: Abnormalities; Animals; Biomarkers; Blood Glucose - metabolism; Body weight; Body Weight - genetics; Carbon 13; Diet; Diet, High-Fat; Dyslipidemia; Eating - genetics; Fatty liver; Food intake; Fructokinases - deficiency; Fructokinases - genetics; Fructokinases - metabolism; Fructose; Fructose Metabolism, Inborn Errors - genetics; Fructose Metabolism, Inborn Errors - metabolism; Fructose-6-phosphate; Fructosephosphates - blood; Glucose; Glucose Intolerance - genetics; Glucose metabolism; Glucose tolerance; Hexokinase; High fat diet; Ingestion; Insulin; Insulin Resistance; Ketohexokinase; Lactic acid; Lipid metabolism; Lipid Metabolism - genetics; Lipids; Lipogenesis; Liver; Metabolic Diseases - genetics; Metabolic Diseases - metabolism; Metabolic disorders; Metabolism; Mice; Mice, Knockout; Muscles; NMR; Nuclear magnetic resonance; Skeletal muscle; Steatosis; Urine; Weight reduction
• ISSN: 0193-1849; 1522-1555
• DOI: 10.1152/ajpendo.00027.2018

Fructose consumption in humans and animals has been linked to enhanced de novo lipogenesis, dyslipidemia, and insulin resistance. Hereditary deficiency of ketohexokinase (KHK), the first enzymatic step in fructose metabolism, leads to essential fructosuria in humans, characterized by elevated levels of blood and urinary fructose following fructose ingestion but is otherwise clinically benign. To address whether KHK deficiency is associated with altered glucose and lipid metabolism, a Khk knockout (KO) mouse line was generated and characterized. NMR spectroscopic analysis of plasma following ingestion of [6- C] fructose revealed striking differences in biomarkers of fructose metabolism. Significantly elevated urine and plasma C-fructose levels were observed in Khk KO vs. wild-type (WT) control mice, as was reduced conversion of C-fructose into plasma C-glucose and C-lactate. In addition, the observation of significant levels of fructose-6-phosphate in skeletal muscle tissue of Khk KO, but not WT, mice suggests a potential mechanism, whereby fructose is metabolized via muscle hexokinase in the absence of KHK. Khk KO mice on a standard chow diet displayed no metabolic abnormalities with respect to ambient glucose, glucose tolerance, body weight, food intake, and circulating trigylcerides, β-hydroxybutyrate, and lactate. When placed on a high-fat and high-fructose (HF/HFruc) diet, Khk KO mice had markedly reduced liver weight, triglyceride levels, and insulin levels. Together, these results suggest that Khk KO mice may serve as a good model for essential fructosuria in humans and that inhibition of KHK offers the potential to protect from diet-induced hepatic steatosis and insulin resistance.