Hypoxanthine Secretion from Human Adipose Tissue and its Increase in Hypoxia

Objective The production of uric acid in murine white adipose tissue (mWAT), and that such production was augmented in obese mice, was recently reported. However, little is known about the secretion of metabolites associated with purine catabolism in human WAT (hWAT). The present study analyzed this...

Full description

Saved in:
Bibliographic Details
Published in:Obesity (Silver Spring, Md.) Md.), 2018-07, Vol.26 (7), p.1168-1178
Main Authors: Nagao, Hirofumi, Nishizawa, Hitoshi, Tanaka, Yoshimitsu, Fukata, Tadafumi, Mizushima, Tsunekazu, Furuno, Masahiro, Bamba, Takeshi, Tsushima, Yu, Fujishima, Yuya, Kita, Shunbun, Funahashi, Tohru, Maeda, Norikazu, Mori, Masaki, Fukusaki, Eiichiro, Shimomura, Iichiro
Format: Article
Language:English
Subjects:
Adipocytes
Body fat
Chemotherapy
Chromatography
Hypoxia
Insulin resistance
Liver
Metabolism
Metabolites
Obesity
Patients
Proteins
Sleep
Solvents
Studies
Uric acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective The production of uric acid in murine white adipose tissue (mWAT), and that such production was augmented in obese mice, was recently reported. However, little is known about the secretion of metabolites associated with purine catabolism in human WAT (hWAT). The present study analyzed this in hWAT. Methods Freshly isolated hWAT and mWAT were cultured. The secretion of metabolites associated with purine catabolism was measured. Tissue distribution profiles of genes associated with purine metabolism and metabolite profiling of adipocytes in hypoxia were analyzed. Results Secretion of hypoxanthine from hWAT was higher than those of xanthine and uric acid. On the other hand, secretion of uric acid was relatively higher than xanthine and hypoxanthine in mWAT. Xanthine oxidoreductase (XOR) mRNA expression levels in hWAT were markedly lower than that in the human liver. In murine tissues, XOR mRNA expression levels in mWAT were comparable with those in the liver. Cultured human adipocytes secreted hypoxanthine, and its secretion was increased under hypoxia. The metabolic analysis of human adipocytes showed that hypoxia increased metabolites associated with de novo biosynthesis of purine nucleotides. Conclusions The present study revealed that hypoxanthine was secreted from human adipose tissue, and the secretion might be increased in local hypoxia.
ISSN:1930-7381
1930-739X
DOI:10.1002/oby.22202