A Survey of Expressed Sequence Tags from the Rainbow Trout (Oncorhynchus Mykiss) Pituitary

The pituitary plays significant roles in the regulation of physiological processes. In the current study, expressed sequence tag data was obtained for 1,920 clones from a normalized mixed-sex pituitary cDNA library. From these 3,840 sequences, a total of 524 contigs were assembled and 1,256 unique s...

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Bibliographic Details
Published in:Animal biotechnology 2007-01, Vol.18 (3), p.213-230
Main Authors: Gahr, Scott A., Rexroad, Caird E., Rise, Matthew L., Hunt, Peter, Koop, Ben
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Contig Mapping
Expressed sequence tag
Expressed Sequence Tags
Female
Gene Expression Profiling
Gene Library
Gene ontology
Male
Molecular Sequence Data
Oncorhynchus mykiss
Oncorhynchus mykiss - genetics
Pituitary
Pituitary Gland - physiology
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Messenger - chemistry
RNA, Messenger - genetics
Sequence Analysis, DNA
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Summary:The pituitary plays significant roles in the regulation of physiological processes. In the current study, expressed sequence tag data was obtained for 1,920 clones from a normalized mixed-sex pituitary cDNA library. From these 3,840 sequences, a total of 524 contigs were assembled and 1,256 unique singletons identified. Assignment of functional annotation was performed through BLAST and gene ontology term assignment. Through in silico comparative mapping homologs were identified for 354 of the unigene sequences. These data provide the first functional information on many of the transcripts present in the rainbow trout pituitary.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495390701337335