UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231

• Published in: Food chemistry 2018-11, Vol.265, p.253-259
• Main Authors: Hamid, Hazrulrizawati Abd, Ramli, Aizi Nor Mazila, Zamri, Normaiza, Yusoff, Mashitah M.
• Format: Article
• Language: English
• Subjects: Antioxidant; Antioxidants - chemistry; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line, Tumor; Cell Survival - drug effects; Chromatography, High Pressure Liquid; Cytotoxicity; Female; Humans; Melastoma decemfidum; Melastomacea malabathricum; Melastomataceae - chemistry; Melastomataceae - metabolism; Phenolic profiles; Phenols - analysis; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plant Leaves - metabolism; Spectrometry, Mass, Electrospray Ionization; UPLC-QTOF/MS
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2018.05.033

•Quercetin-3-O-α-l-arabinofuranoside was abundant in M. malabathricum.•6-Hydroxykaempferol-3-O-glucoside was abundant in M. decenfidum.•M. decenfidum possessed higher TPC, TFC and antioxidant activity.•M. malabathricum was more cytotoxic than M. decenfidum towards MDA-MB-231 cell. Eleven compounds were identified during profiling of polyphenols by UPLC-QTOF/MS. In abundance was quercetin-3-O-α-l-arabinofuranoside in M. malabathricum ethanolic leaves extract while 6-hydroxykaempferol-3-O-glucoside was present in the leaves extract of M. decenfidum (its rare variety). TPC and TFC were significantly higher in M. decemfidum extract than M. malabathricum extract. During DPPH, FRAF and β-carotene bleaching assays, M. decemfidum extract exhibited greater antioxidant activity compared to M. malabathricum extract. Effect of M. malabathricum and M. decemfidum extracts on viability of MDA-MB-231 cell at concentrations 6.25–100 μg/mL were evaluated for 24, 48 and 72 h. After 48 and 72 h treatment, M. malabathricum and M. decemfidum leaves extracts exhibited significant activity in inhibiting MDA-MB-231 cancer cell line with M. malabathricum extract being more cytotoxic. M. malabathricum and M. imbricatum serves as potential daily dietary source of natural phenolics and to improve chemotherapeutic effectiveness.