Immobilization of laccase-ABTS system for the development of a continuous flow packed bed bioreactor for decolorization of textile effluent

Decolourization of textile effluent is always being a problem of major concern for the safe disposal of effluent water in river stream. Due to intense colour of the effluent water, even after the treatment, disposal of the water in river stream is under strict environmental regulation. Laccase, a na...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2018-10, Vol.117, p.1093-1100
Main Authors: Sondhi, Sonica, Kaur, Randhir, Kaur, Supreet, Kaur, Palki Sahib
Format: Article
Language:English
Subjects:
Alginates
Benzothiazoles - chemistry
Biodegradation, Environmental
Bioreactors
Biotransformation
Coloring Agents
Enzyme Stability
Enzymes, Immobilized
Glucuronic Acid
Hexuronic Acids
Hydrogen-Ion Concentration
Immobilization
Industrial Waste
Laccase
Laccase - chemistry
Laccase - metabolism
LMS
Microspheres
Packed bed bioreactor
Sulfonic Acids - chemistry
Temperature
Textile effluent
Textile Industry
Waste Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Decolourization of textile effluent is always being a problem of major concern for the safe disposal of effluent water in river stream. Due to intense colour of the effluent water, even after the treatment, disposal of the water in river stream is under strict environmental regulation. Laccase, a natural oxidase, have the miraculous power to decolourize different dyes with or without mediators. However, stability and cost of implementation of the enzyme at industrial conditions make the entire process unfeasible at industrial scale. In the present study, a highly thermo-alkali-stable, metal, surfactant and organic solvent tolerant laccase from Bacillus sp. MSK-01 was immobilized in Cu-alginate bead with ABTS mediator under standardized conditions. A continuous flow packed bed bioreactor was formulated to develop a continuous method of the treatment of effluent water. Results showed 66% reduction in the colour of the effluent. UV-VIS spectrum analysis of the treated and untreated samples showed the formation of the degradation products of dyes due to the action of laccase. The developed process can be made useful for on site industrial application.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2018.06.007