Multilocus sequence analysis of Vibrionaceae isolated from farmed amberjack and the development of a multiplex PCR assay for the detection of pathogenic species

Since 2011, high mortality rates and symptoms consistent with vibriosis have been observed in farmed amberjack (Seriola dumerili) in Japan. To identify 41 strains isolated from diseased amberjack, a multilocus sequence analysis using nine concatenated genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA,...

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Bibliographic Details
Published in:Journal of fish diseases 2018-08, Vol.41 (8), p.1295-1301
Main Authors: Nishiki, Issei, Minami, Takayuki, Murakami, Ayana, Hoai, Truong Dinh, Fujiwara, Atushi
Format: Article
Language:English
Subjects:
amberjack
Aquaculture
Bacteria
Detection
Diagnostic systems
DNA
DNA-directed RNA polymerase
Epidemics
Farms
Genes
Identification
Methods
Microbiological strains
multilocus sequence analysis
multiplex PCR
Multiplexing
Nucleotide sequence
Pathogens
PCR
Photobacterium damselae subsp. damselae
RecA protein
rRNA 16S
Sequencing
Species
Strains (organisms)
Symptoms
Vibrio anguillarum
Vibrio harveyi
Vibriosis
Waterborne diseases
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Summary:Since 2011, high mortality rates and symptoms consistent with vibriosis have been observed in farmed amberjack (Seriola dumerili) in Japan. To identify 41 strains isolated from diseased amberjack, a multilocus sequence analysis using nine concatenated genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, topA and 16S rRNA) was conducted. Twenty‐seven strains were identified as Vibrio harveyi, suggesting an epidemic of V. harveyi infection in amberjack farms. Other strains were identified as Vibrio anguillarum, Vibrio owensii and Photobacterium damselae subsp. damselae. To develop an efficient diagnostic method for vibriosis in amberjack, a multiplex PCR system was developed to identify V. anguillarum, V. harveyi and P. damselae subsp. damselae. The method successfully discriminated between these three bacterial species, with amplification products of 350 bp for V. anguillarum, 545 bp for V. harveyi and 887 bp for P. damselae subsp. damselae and can be used for diagnosis in aquaculture farms.
ISSN:0140-7775
1365-2761
DOI:10.1111/jfd.12823