Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report

In vitro studies have demonstrated that GM-CSF in combination with other stimulatory factors induces a microbicidal response that control T. gondii infection. We assessed whether GM-CSF alone can control T. gondii replication in murine microglial cultures. Microglia were collected and cultured with...

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Bibliographic Details
Published in:Microbes and infection 2018-06, Vol.20 (6), p.385-390
Main Authors: Pimenta, Tamirys Simão, Chaves, Natalie Ferreira, Rodrigues, Ana Paula Drummond, Diniz, Cristovam Wanderley Picanço, DaMatta, Renato Augusto, Diniz Junior, José Antônio Picanço
Format: Article
Language:English
Subjects:
Cytokines
GM-CSF
Microglia
Nitric oxide
Superoxide
T. gondii
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Summary:In vitro studies have demonstrated that GM-CSF in combination with other stimulatory factors induces a microbicidal response that control T. gondii infection. We assessed whether GM-CSF alone can control T. gondii replication in murine microglial cultures. Microglia were collected and cultured with or without GM-CSF and the half of each group was infected with T. gondii. We determined the T. gondii infectivity, cytokines levels, NO and superoxide detection. GM-CSF alone primes microglia, which after infection induces the production of TNF-α and IL-6, leading to NO and superoxide production, without any stimulus from IL-12p70 and IFN-γ.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2018.05.006