Direct Monitoring of Fucosylated Glycopeptides of Alpha‐Fetoprotein in Human Serum for Early Hepatocellular Carcinoma by Liquid Chromatography–Tandem Mass Spectrometry with Immunoprecipitation

Purpose: Alpha‐fetoprotein (AFP) is a widely used serological marker that is associated with hepatocellular carcinoma (HCC). Although the level of AFP is increased in HCC, its sensitivity for diagnosis is poor because AFP levels are also increased in liver diseases. Changes in glycoform, especially...

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Bibliographic Details
Published in:Proteomics. Clinical applications 2018-11, Vol.12 (6), p.e1800062-n/a
Main Authors: Kim, Kwang Hoe, Lee, Soo‐Youn, Hwang, Heeyoun, Lee, Ju Yeon, Ji, Eun Sun, An, Hyun Joo, Kim, Jin Young, Yoo, Jong Shin
Format: Article
Language:English
Subjects:
alpha‐fetoprotein
Amino acid sequence
Antibiotics
Chromatography
Cirrhosis
Design of experiments
Exo-a-sialidase
Experimental design
fucosylated glycopeptide
Glycan
Glycopeptides
Hepatocellular carcinoma
Immunoprecipitation
Liquid chromatography
Liver
Liver cancer
Liver cirrhosis
Liver diseases
Mass spectrometry
Mass spectroscopy
Monitoring
parallel reaction monitoring
Scientific imaging
Sensitivity
Spectroscopy
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Summary:Purpose: Alpha‐fetoprotein (AFP) is a widely used serological marker that is associated with hepatocellular carcinoma (HCC). Although the level of AFP is increased in HCC, its sensitivity for diagnosis is poor because AFP levels are also increased in liver diseases. Changes in glycoform, especially fucosylation, have been reported to be associated with the development of HCC. Experimental design: The authors introduce the monitoring of fucosylated glycopeptides by liquid chromatography (LC)‐mass spectrometry (MS) combined with immunoprecipitation, where glycan‐cleaved fragments with an amino acid sequence are used as transitions. Furthermore, neuraminidase for desialylation is useful to improve the MS detection limit (limit of detection [LOD]
ISSN:1862-8346
1862-8354
DOI:10.1002/prca.201800062