Direct evidence for the up-regulation of Vps34 regulated by PKCγ during short-term treatment with morphine

In this study, we investigated whether PKCγ could be associated with functional changes of vacuolar protein sorting 34 (Vps34) during morphine treatment using primary cultures of cerebral cortical neurons from mice. The immunoprecipitation analysis showed that p‐PKCγ and Vps34 are present together i...

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Bibliographic Details
Published in:Synapse (New York, N.Y.) N.Y.), 2009-05, Vol.63 (5), p.365-368
Main Authors: Shibasaki, Masahiro, Kurokawa, Kazuhiro, Katsura, Masashi, Ohkuma, Seitaro
Format: Article
Language:English
Subjects:
PI 3-kinase
synaptic plasticity
trafficking
μ-opioid receptor
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Summary:In this study, we investigated whether PKCγ could be associated with functional changes of vacuolar protein sorting 34 (Vps34) during morphine treatment using primary cultures of cerebral cortical neurons from mice. The immunoprecipitation analysis showed that p‐PKCγ and Vps34 are present together in molecular complexes. The treatment with morphine increases PKCγ and Vps34 levels. Phosphorylation of PKCγ increased Vps34 level. The inhibition of morphine‐induced increase in PKCγ phosphorylation reduced Vps34 level. These results indicates that opioid receptor activation increases PKCγ phosphorylation in the neurons and, in turn, upregulates Vps34 during short‐term treatment with neurons. Synapse 63:365–368, 2009. © 2009 Wiley‐Liss, Inc.
ISSN:0887-4476
1098-2396
DOI:10.1002/syn.20612