Inhibition of inflammatory mediators by polyphenolic plant extracts in human intestinal Caco-2 cells

• Published in: Food and chemical toxicology 2009-06, Vol.47 (6), p.1221-1230
• Main Authors: Romier-Crouzet, Béatrice, Van De Walle, Jacqueline, During, Alexandrine, Joly, Aurélie, Rousseau, Charline, Henry, Olivier, Larondelle, Yvan, Schneider, Yves-Jacques
• Format: Article
• Language: English
• Subjects: Amidines; anti-inflammatory activity; Antioxidants - isolation & purification; Antioxidants - pharmacology; Biological and medical sciences; Caco-2 Cells; cell culture; Cell Survival - drug effects; cocoa beans; Copper - chemistry; cytokines; Dinoprostone - biosynthesis; Enzyme Activation - drug effects; Erk1/2; Flavonoids - isolation & purification; Flavonoids - pharmacology; Free Radicals - chemistry; General pharmacology; grape seeds; Hemolysis - drug effects; human cell lines; Humans; Il-8 production; immune response; inflammation; Inflammation Mediators - antagonists & inhibitors; interleukin-8; Interleukin-8 - biosynthesis; Lipoproteins, LDL - chemistry; luciferase; mangosteens; Medical sciences; mitogen-activated protein kinase; Mitogen-Activated Protein Kinases - metabolism; NF-kappa B - antagonists & inhibitors; NF-κB pathway; Nitric Oxide - biosynthesis; nitric oxide synthase; NO production; Oxidation-Reduction; PGE2 secretion; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenols - isolation & purification; Phenols - pharmacology; plant extracts; Plant Extracts - pharmacology; Plant poisons toxicology; Plants - chemistry; Polyphenolic extracts; Polyphenols; pomegranates; prostaglandins; Quercus; sugarcane; Thiobarbituric Acid Reactive Substances; Toxicology; transcription factors; Vitaceae
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2009.02.015

The mitogen-activated protein kinases (MAPK) and nuclear factor κB (NF-κB) are involved in transduction cascades that play a key role in inflammatory response. We tested the ability of preselected natural polyphenolic extracts (grape seed, cocoa, sugar cane, oak, mangosteen and pomegranate) to modulate intestinal inflammation using human intestinal Caco-2 cells treated for 4h with these extracts and then stimulated by cytokines for 24 or 48h. The effect of polyphenolic extracts, at 50μmol of gallic acid equivalent/l, was investigated on inflammation-related cellular events: (i) NF-κB activity (cells transfected with a NF-κB-luciferase construct), (ii) activation of Erk1/2 and JNK (western blotting), (iii) secretion of interleukin 8 (IL-8) (ELISA), (iv) secretion of prostaglandin (PG) E2 (ELISA), (v) production of NO (Griess method). Results show that: (i) sugar cane, oak and pomegranate extracts inhibited NF-κB activity (from 1.6 to 1.9-fold) (P