Extracts of Cynomorium songaricum protect SK-N-SH human neuroblastoma cells against staurosporine-induced apoptosis potentially through their radical scavenging activity

In traditional Chinese medicine a number of herbs are used to alleviate the symptoms of aging, among them the stems of Cynomorium songaricum, Cynomoriaceae. This study evaluated the protective effect of different extracts of C. songaricum on staurosporine-induced apoptotic cell death in SK-N-SH neur...

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Published in:Phytotherapy research 2009-02, Vol.23 (2), p.257-261
Main Authors: Lu, Yi, Wang, Qingguo, Melzig, Matthias F, Jenett-Siems, Kristina
Format: Article
Language:English
Subjects:
Aging - drug effects
Apoptosis - drug effects
Biological and medical sciences
Cell Line, Tumor
Cell Survival
Cynomoriaceae
Cynomorium songaricum
Free Radical Scavengers - pharmacology
General pharmacology
Humans
Magnoliopsida - chemistry
Medical sciences
Medicine, Chinese Traditional
neuroprotection
Neuroprotective Agents - pharmacology
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - pharmacology
staurosporine
Staurosporine - toxicity
superoxide anion
Superoxides - metabolism
traditional Chinese medicine
xanthine oxidase
Xanthine Oxidase - metabolism
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Summary:In traditional Chinese medicine a number of herbs are used to alleviate the symptoms of aging, among them the stems of Cynomorium songaricum, Cynomoriaceae. This study evaluated the protective effect of different extracts of C. songaricum on staurosporine-induced apoptotic cell death in SK-N-SH neuroblastoma cells. Staurosporine (100 nm) reduced cell viability to about 55%. The ethyl acetate fraction of C. songaricum significantly attenuated staurosporine-induced cell death at concentrations of 100 and 10 μg/mL. On the other hand, the dichloromethane as well as water fractions showed no protective effects. In order to further analyse the protective mode of action, the superoxide anion scavenging activity of two extracts was evaluated in a xanthine/xanthine oxidase system. In this system, the EtOAc extract showed a good scavenging activity (IC₅₀ value 2.9 μg/mL) without inhibition of xanthine oxidase. In conclusion, the results prove the neuroprotective activity of C. songaricum extracts in vitro, thus supporting its traditional use. Copyright © 2008 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.2605