Resveratrol promotes neuroprotection and attenuates oxidative and nitrosative stress in the small intestine in diabetic rats

• Published in: Biomedicine & pharmacotherapy 2018-09, Vol.105, p.724-733
• Main Authors: Ferreira, Paulo Emilio Botura, Beraldi, Evandro José, Borges, Stephanie Carvalho, Natali, Maria Raquel Marçal, Buttow, Nilza Cristina
• Format: Article
• Language: English
• Subjects: Animals; Diabetes; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - drug therapy; Diabetic Neuropathies - metabolism; Diabetic Neuropathies - prevention & control; Intestine, Small - drug effects; Intestine, Small - innervation; Intestine, Small - metabolism; Male; Myenteric plexus; Myenteric Plexus - drug effects; Neuroprotection; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; Nitrosative stress; Nitrosative Stress - drug effects; Oxidative stress; Oxidative Stress - drug effects; Rats, Wistar; Resveratrol; Stilbenes - pharmacology; Stilbenes - therapeutic use; Streptozocin
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2018.06.030

[Display omitted] •Nitrosative and oxidative stress is cause of the damages to the myenteric plexus.•RSV presented antioxidant effect in the intestinal wall in diabetic rats.•RSV promoted neuroprotection in the myenteric plexus in diabetic rats.•RSV may be promising for the treatment of damage in the ENS caused by diabetic. Damages to the enteric nervous system caused by diabetes mellitus (DM) are frequently attributed to oxidative and nitrosative stress. We aimed to investigate the effect of Resveratrol (RSV) (10 mg/kg) on oxidative and nitrosative stress in the intestinal wall and morphoquantitative aspects of the myenteric plexus of the duodenum, jejunum and ileum in diabetic rats. Twenty-four rats were distributed into four groups (n = 6/group): control (C group), control treated with RSV (CR group), diabetic (D group), and diabetic treated with RSV (DR group) for 120 days. Immunohistochemical staining techniques for the general neuronal population, nitrergic and calretinin neuronal subpopulations, enteric glial cells and glial fibrillary acid protein were performed in the myenteric plexus. Furthermore, parameters of oxidative and nitrosative stress were analyzed in the intestinal wall. RSV attenuated oxidative and nitrosative stress and prevented neuronal loss and hypertrophy of the HuC/D-IR, nNOS-IR and CALR-IR neuronal subpopulations in the DR group compared with the D group (P