Effect of human adipose-derived stem cells on pressure ulcer healing in mouse

To investigate the effect of human adipose-derived stem cells (hADSCs) on pressure ulcers in mouse. The subcutaneous adipose tissue from voluntary donation was harvested. Then the hADSCs were isolated and cultured by mechanical isolation combined with typeⅠcollagenase digestion. The 3rd generation c...

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Bibliographic Details
Published in:Zhongguo xiu fu chong jian wai ke za zhi 2018-06, Vol.32 (6), p.726-735
Main Authors: Deng, Chengliang, Liu, Zhiyuan, Yao, Yuanzhen, Liu, Ruichi, Wei, Zairong, Wang, Dali
Format: Article
Language:Chinese
Subjects:
Adipocytes
Animals
Humans
Mice
Mice, Inbred C57BL
Pressure Ulcer - therapy
Stem Cells
Wound Healing
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Summary:To investigate the effect of human adipose-derived stem cells (hADSCs) on pressure ulcers in mouse. The subcutaneous adipose tissue from voluntary donation was harvested. Then the hADSCs were isolated and cultured by mechanical isolation combined with typeⅠcollagenase digestion. The 3rd generation cells were identified by osteogenic, adipogenic, chondrogenic differentiations and flow cytometry. The platelet rich plasma (PRP) from peripheral blood donated by healthy volunteers was prepared by centrifugation. The pressure ulcer model was established in 45 C57BL/6 mice by two magnets pressurized the back skin, and randomly divided into 3 groups ( =15). The wounds were injected with 100 μL of hADSCs (1×10 cells) transfected with a green fluorescent protein (GFP)-carrying virus, 100 μL human PRP, and 100 μL PBS in hADSCs group, PRP group, and control group, respectively. The wound healing was observed after injection. The wound healing rate was calculated on the 5th, 9th, and 13th days. On the 5th, 11th, and 21st
ISSN:1002-1892
DOI:10.7507/1002-1892.201801031