Quantitative differential phase contrast imaging at high resolution with radially asymmetric illumination

Half-circle illumination-based differential phase contrast (DPC) microscopy has been utilized to recover phase images through a pair of images along multiple axes. Recently, the half-circle based DPC using 12-axis measurements significantly provides a circularly symmetric phase transfer function to...

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Bibliographic Details
Published in:Optics letters 2018-06, Vol.43 (12), p.2973-2976
Main Authors: Lin, Yu-Zi, Huang, Kuang-Yuh, Luo, Yuan
Format: Article
Language:English
Subjects:
Illumination
Image contrast
Image resolution
Microscopes
Microspheres
Phase contrast
Recovery
Transfer functions
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Summary:Half-circle illumination-based differential phase contrast (DPC) microscopy has been utilized to recover phase images through a pair of images along multiple axes. Recently, the half-circle based DPC using 12-axis measurements significantly provides a circularly symmetric phase transfer function to improve accuracy for more stable phase recovery. Instead of using half-circle-based DPC, we propose a new scheme of DPC under radially asymmetric illumination to achieve circularly symmetric phase transfer function and enhance the accuracy of phase recovery in a more stable and efficient fashion. We present the design, implementation, and experimental image data demonstrating the ability of our method to obtain quantitative phase images of microspheres, as well as live fibroblast cell samples.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.43.002973