Fast repair of oxidative DNA damage by phenylpropanoid glycosides and their analogues

The repair activities and reaction mechanisms of phenylpropanoid glycosides (PPGs) and their analogues, isolated from Chinese folk medicinal herbs, towards oxidative DNA damage were studied with pulse radiolytic technique. On pulse irradiation of nitrogen-saturated 4 mM poly C aqueous solution conta...

Full description

Saved in:
Bibliographic Details
Published in:Mutagenesis 2008-01, Vol.23 (1), p.19-26
Main Authors: Shi, Yimin, Wang, Wengfeng, Huang, Chungyang, Jia, Zhongjian, Yao, Side, Zheng, Rongliang
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA - metabolism
DNA Damage
DNA Repair
DNA, Single-Stranded - metabolism
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Glycosides - chemistry
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Oxidation-Reduction
Phenylpropionates - chemistry
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The repair activities and reaction mechanisms of phenylpropanoid glycosides (PPGs) and their analogues, isolated from Chinese folk medicinal herbs, towards oxidative DNA damage were studied with pulse radiolytic technique. On pulse irradiation of nitrogen-saturated 4 mM poly C aqueous solution containing one of the tested polyphenols, 40 mM K2S2O8 and 200 mM t-BuOH, the transient absorption spectrum of the oxidative radical of poly C decays with the concurrent formation of the phenoxyl radical of the tested polyphenols within several tens of microseconds after the electron pulse irradiation. The result indicated that there was a repair reaction between oxidative radical of poly C and the tested polyphenols. The repair activities also were observed for the tested polyphenols towards the radical cations of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The rate constants were determined to be 3.7–6.4 × 109, 4.8–5.5 × 108 and 8.8–10.3 × 108 M−1·sec−1 for the repair reactions of oxidative radical of poly C and radical cations of ssDNA and dsDNA, respectively. The result of this study together with those of our previous studies demonstrates that PPGs and their analogues can fast repair not only the damage of deoxynucleoside and deoxynucleotide but also the damage of integral DNA, with the latter being closer to a cellular condition.
ISSN:0267-8357
1464-3804
DOI:10.1093/mutage/gem028