PyA-cluster system for the detection and imaging of miRNAs in living cells through double-three-way junction formation

Herein, we describe an extended version of a fluorescence probe for detecting miRNAs through the novel application of a PyA-cluster system. By testing various (CG)n sequences in the middle of the oligonucleotide strand of the probe, we obtained an optimal sequence that formed a double-three-way-junc...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2018-01, Vol.54 (54), p.7471-7474
Main Authors: Ro, Jong Jin, Lee, Ha Jung, Kim, Byeang Hyean
Format: Article
Language:eng ; jpn
Subjects:
Cells (biology)
Clusters
Fluorescent indicators
Sequences
Target detection
Online Access:Get full text
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Summary:Herein, we describe an extended version of a fluorescence probe for detecting miRNAs through the novel application of a PyA-cluster system. By testing various (CG)n sequences in the middle of the oligonucleotide strand of the probe, we obtained an optimal sequence that formed a double-three-way-junction structure, with two PyA units positioned close together, in the presence of the target miRNA. This system readily detected the locations of target miRNAs in living cells and allowed visualization of structural changes through variations in the color of the fluorescence.
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc03982h