Excess and deficient omega-3 fatty acid during pregnancy and lactation cause impaired neural transmission in rat pups

• Published in: Neurotoxicology and teratology 2008-03, Vol.30 (2), p.107-117
• Main Authors: Church, M.W, Jen, K.-L.C, Dowhan, L.M, Adams, B.R, Hotra, J.W
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn - physiology; Auditory brainstem response (ABR); Biological and medical sciences; Body Temperature - drug effects; Body Weight - physiology; Data Interpretation, Statistical; Diet; Docosahexanoic acid (DHA); Dose-Response Relationship, Drug; Eicosapentanoic acid (EPA); Emergency; Fatty Acids - analysis; Fatty Acids, Omega-3 - physiology; Fatty Acids, Omega-3 - toxicity; Female; Fetal programming; Fish oil; Hearing - drug effects; Lactation; Lactation - drug effects; Lactation - physiology; Medical Education; Medical sciences; Milk - chemistry; Omega-3 fatty acids (ω-3 FA); Omega-6 fatty acids (ω-6 FA); Postnatal; Pregnancy; Prenatal; Rats; Rats, Wistar; Synaptic Transmission - drug effects; Synaptic Transmission - physiology; Toxicology
• ISSN: 0892-0362; 1872-9738
• DOI: 10.1016/j.ntt.2007.12.008

Abstract Omega-3 fatty acids (ω-3 FA) consumption during pregnancy and lactation is beneficial to fetal and infant growth and may reduce the severity of preterm births. Thus, scientists and clinicians are recommending increasingly higher ω-3 FA doses for pregnant women and nursing babies for advancing the health of preterm, low birth weight, and normal babies. In contrast, some studies report that over-supplementation with ω-3 FA can have adverse effects on fetal and infant development by causing a form of nutritional toxicity. Our goal was to assess the effects of ω-3 FA excess and deficiency during pregnancy and lactation on the offspring's neural transmission as evidenced by their auditory brainstem responses (ABR). Female Wistar rats were given one of three diets from day 1 of pregnancy through lactation. The three diets were the Control ω-3 FA condition (ω-3/ω-6 ratio ∼ 0.14), the Deficient ω-3 FA condition (ω-3/ω-6 ratio ∼ 0%) and the Excess ω-3 FA condition (ω-3/ω-6 ratio ∼ 14.0). The Control diet contained 7% soybean oil, whereas the Deficient diet contained 7% safflower oil and the Excess diet contained 7% fish oil. The offspring were ABR-tested on postnatal day 24. The rat pups in the Excess group had prolonged ABR latencies in comparison to the Control group, indicating slowed neural transmission times. The pups in the Excess group also showed postnatal growth restriction. The Deficient group showed adverse effects that were milder than those seen in the Excess group. Milk fatty acid profiles reflected the fatty acid profiles of the maternal diets. In conclusion, excess or deficient amounts of ω-3 FA during pregnancy and lactation adversely affected the offspring's neural transmission times and postnatal thriving. Consuming either large or inadequate amounts of ω-3 FA during pregnancy and lactation seems inadvisable because of the potential for adverse effects on infant development.