Binding of Mucin by E. coli from Human Gut

Cells of E. coli isolates from the gut of healthy volunteers ( N =5) and patients with Crohn’s disease ( N =5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding was evaluated by the decrease in optical absorpti...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2018-06, Vol.165 (2), p.235-238
Main Authors: Vakhrusheva, T. V., Baikova, Yu. P., Balabushevich, N. G., Gusev, S. A., Lomakina, G. Yu, Sholina, E. A., Moshkovskaya, M. A., Shcherbakov, P. L., Pobeguts, O. V., Mikhal’chik, E. V.
Format: Article
Language:English
Subjects:
Analysis
Bacteria
Biomedical and Life Sciences
Biomedicine
Cell Biology
Confocal microscopy
Crohn's disease
E coli
Internal Medicine
Laboratory Medicine
Mucin
Pathology
Sodium chloride
Zeta potential
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Summary:Cells of E. coli isolates from the gut of healthy volunteers ( N =5) and patients with Crohn’s disease ( N =5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding was evaluated by the decrease in optical absorption of mucin solution at 214 nm after incubation with bacteria. Detailed analysis of mucin binding by one of isolates showed that during incubation of 0.09 mg/ml bacteria in 0.15 M NaCl containing 0.1 mg/ml mucin at 25 o C, maximum binding was reached in 30 min, while in the presence of 14 mM α-methyl mannoside, mucin binding decreased by 46% ( p
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-018-4137-3