Detection of Genistein as an Estrogenic Contaminant of River Water in Osaka

• Published in: Environmental science & technology 2004-12, Vol.38 (23), p.6424-6429
• Main Authors: Kawanishi, Masanobu, Takamura-Enya, Takeji, Ermawati, Rahyani, Shimohara, Chiaki, Sakamoto, Michiyo, Matsukawa, Kazuki, Matsuda, Tomonari, Murahashi, Tsuyoshi, Matsui, Saburo, Wakabayashi, Keiji, Watanabe, Tetsushi, Tashiro, Yutaka, Yagi, Takashi
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Chromatography, High Pressure Liquid; Contamination; Ecotoxicology, biological effects of pollution; Environmental impact; Estrogens; Estrogens - chemistry; Estrogens - urine; Fresh water environment; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Genistein - urine; Humans; Japan; Receptors, Estrogen - drug effects; Receptors, Estrogen - metabolism; Rivers; Rivers - chemistry; Waste Disposal, Fluid; Water Pollutants, Chemical - analysis
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es049764v

The estrogenic activity in water at various localities on Lake Biwa−Yodo River, a representative watershed in Japan, was measured using a recombinant yeast that expresses the human estrogen receptor. The yeast bioassay revealed that the activities of 13 water samples had an average value of 14 pmol/L (3.8 ng/L) (17β-estradiol equivalent) with a very wide range from 0 to 72 pmol/L (0−19.6 ng/L), and two of the samples had prominent levels of activity (72 pmol/L (19.6 ng/L) and 56 pmol/L (15.2 ng/L)). We analyzed these two samples with instrumental approaches. A high-performance liquid chromatogram profile showed that the strong activity in one sample, which was collected just downstream of a sewage-treatment plant, would be due to 17β-estradiol and estrone, whose source is considered to be human urine contained in the effluent of the plant. The activity in the other sample, which was obtained from a tributary river in a primarily residential area with some industrial development (i.e., Osaka City), however, did not correspond to 17β-estradiol, estrone, or synthetic chemicals known as estrogenic. Analysis of a fraction with estrogenic activity by liquid chromatography−mass spectrometry (LC−MS) provided evidence that the activity in the water sample resulted from the presence of genistein, an isoflavone compound of plant origin.