Isoprene synthesis protects transgenic tobacco plants from oxidative stress

• Published in: Plant, cell and environment cell and environment, 2009-05, Vol.32 (5), p.520-531
• Main Authors: VICKERS, CLAUDIA E., POSSELL, MALCOLM, COJOCARIU, CRISTIAN I., VELIKOVA, VIOLETA B., LAOTHAWORNKITKUL, JULLADA, RYAN, ANNETTE, MULLINEAUX, PHILIP M., NICHOLAS HEWITT, C.
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Biological and medical sciences; Butadienes; Fundamental and applied biological sciences. Psychology; Hemiterpenes - biosynthesis; Hot Temperature; isoprene synthase; Nicotiana - drug effects; Nicotiana - genetics; Nicotiana - metabolism; oxidative damage; Oxidative Stress; ozone; Ozone - pharmacology; Pentanes; Photosynthesis; Plants, Genetically Modified - drug effects; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; reactive oxygen species; Reactive Oxygen Species - metabolism; thermotolerance
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/j.1365-3040.2009.01946.x

ABSTRACT Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high‐temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene‐emitting plants were highly resistant to ozone‐induced oxidative damage compared with their non‐emitting azygous controls. In ozone‐treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene‐emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non‐emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene‐emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.