Comparison of growth factor and interleukin content of adult peripheral blood and cord blood serum eye drops for cornea and ocular surface diseases

• Published in: Transfusion and apheresis science 2018-08, Vol.57 (4), p.549-555
• Main Authors: Buzzi, M., Versura, P., Grigolo, B., Cavallo, C., Terzi, A., Pellegrini, M., Giannaccare, G., Randi, V., Campos, E.C.
• Format: Article
• Language: English
• Subjects: Adult; Adult peripheral blood; Cord blood; Cornea - drug effects; Corneal healing; Eye Infections - drug therapy; Female; Fetal Blood; Growth factors; Health technology assessment; Humans; Intercellular Signaling Peptides and Proteins - metabolism; Interleukins; Interleukins - metabolism; Male; Middle Aged; Ophthalmic Solutions - therapeutic use; Prospective Studies; Serum; Serum eye drops
• ISSN: 1473-0502; 1878-1683
• DOI: 10.1016/j.transci.2018.06.001

Various blood-derived products have been proposed for the topical treatment of ocular surface diseases. The aim of the study was to compare the different content of Growth Factors (GFs) and Interleukins (ILs) in peripheral blood (PB-S) and Cord Blood (CB-S) sera. Sera were obtained from 105 healthy adult donors (PB-S) and 107 umbilical/placental veins at the time of delivery (CB-S). The levels of epithelial-GF (EGF), fibroblast-GF (FGF), platelet-derived-GF (PDGF), insulin-GF (IGF), transforming-GF alpha (TGF-α,) and beta 1-2-3 (TGF-β1-β2-β3), vascular endothelial-GF (VEGF), nerve-GF (NGF), Interleukin (IL)-1β,IL-4,IL-6,IL-10, and IL-13 were assessed by Bio-Plex Protein Array System (Bio-Rad Laboratories, CA, USA). The Mann-Whitney test for unpaired data was applied to compare GFs and ILs levels in the two sources. The associations among each GF/IL level and the obstetric data for CB-S and hematological characteristics for PB-S were also investigated. The levels of EGF, TGF-α, TGF-β2, FGF, PDGF, VEGF, NGF, IL-1B, IL-4, IL-6, IL-10, and IL-13 were significantly higher in CB-S compared to PB-S. Conversely, the levels of IGF-1, IGF-2, and TGF-β1 were significantly higher in PB-S. The female sex and the weight of the child showed a significant association in predicting EGF and PDGF levels. A significantly different content in those GFs and ILs was demonstrated in the two blood sources. Since each GF/IL selectively regulates different cellular processes involved in corneal healing, the use of PB-S or CB-S should be chosen on the basis of the cellular mechanism to be promoted in each clinical case.