Differences in Cellular Composition of Peripheral Blood Stem Cell Grafts from Healthy Stem Cell Donors Mobilized with Either Granulocyte Colony-Stimulating Factor (G-CSF) Alone or G-CSF and Plerixafor

• Published in: Biology of blood and marrow transplantation 2018-11, Vol.24 (11), p.2171-2177
• Main Authors: Teipel, Raphael, Oelschlägel, Uta, Wetzko, Katrin, Schmiedgen, Maria, Kramer, Michael, Rücker-Braun, Elke, Hölig, Kristina, von Bonin, Malte, Heidrich, Katharina, Fuchs, Anke, Ordemann, Rainer, Kroschinsky, Frank, Bornhäuser, Martin, Hütter, Gero, Schmidt, Helmuth, Ehninger, Gerhard, Schetelig, Johannes, Heidenreich, Falk
• Format: Article
• Language: English
• Subjects: Anti-HIV Agents - pharmacology; Anti-HIV Agents - therapeutic use; Cell composition; Female; Granulocyte colony-stimulating factor; Granulocyte Colony-Stimulating Factor - pharmacology; Granulocyte Colony-Stimulating Factor - therapeutic use; Healthy Volunteers; Hematopoietic Stem Cell Mobilization - methods; Heterocyclic Compounds - pharmacology; Heterocyclic Compounds - therapeutic use; Humans; Leukapheresis; Leukapheresis - methods; Male; Peripheral Blood Stem Cell Transplantation - methods; Peripheral Blood Stem Cells - metabolism; Plerixafor; Stem cell mobilization; Tissue Donors; Transplants - transplantation
• ISSN: 1083-8791; 1523-6536
• DOI: 10.1016/j.bbmt.2018.06.023

•Grafts for hematopoietic stem cell transplantation collected from healthy donors mobilized with G-CSF and plerixafor differ significantly in their cellular composition compared with grafts from the same donors mobilized with G-CSF alone.•Leukapheresis samples mobilized with additional plerixafor showed a significant increase of total nucleated cells, including B cells, CD4+ and CD8+ T cells, and CD34+ hematopoietic stem and progenitor cells.•Increased numbers of regulatory cells such as Tregs and plasmacytoid dendritic cells in the G-CSF and plerixafor mobilized graft provide a rationale for further studies to investigate the impact on GVHD and outcome of transplantation. This study was conducted to characterize and compare peripheral blood stem cell grafts from healthy donors who underwent granulocyte colony-stimulating factor (G-CSF) mobilization and subsequently received 1 dose of plerixafor after insufficient stem cell yields were achieved at the first apheresis. Aliquots from 35 donors were collected from the first apheresis after mobilization with G-CSF alone and from the second apheresis after additional plerixafor administration. Samples were freshly analyzed for cellular subsets by 8-color flow cytometry. Leukapheresis samples mobilized with additional plerixafor showed a significant increase of total nucleated cells, including B cells, CD4+ and CD8+ T cells, and CD34+ hematopoietic stem and progenitor cells. Absolute numbers of plasmacytoid dendritic cells were also significantly increased, whereas no changes were detected for myeloid dendritic cells. Furthermore, absolute numbers of regulatory T cells increased, with naive CD45RA+ regulatory T cells showing the highest rise. Finally, strikingly higher numbers of myeloid-derived suppressor cells were detected in the plerixafor and G-CSF–mobilized graft. The mobilization of peripheral stem cells in healthy donors with G-CSF and plerixafor led to a significant difference in cellular graft composition compared with G-CSF alone. The clinical impact of the different cell composition for the graft recipient warrants further clinical investigation.