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Stimulation of calvarial bone healing with human bone marrow stromal cells versus inhibition with adipose-tissue stromal cells on nanostructured β-TCP-collagen
[Display omitted] Bioactive functional scaffolds are essential for support of cell-based strategies to improve bone regeneration. Adipose-tissue-derived-stromal cells (ASC) are more accessible multipotent cells with faster proliferation than bone-marrow-derived-stromal-cells (BMSC) having potential...
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| Published in: | Acta biomaterialia 2018-08, Vol.76, p.135-145 |
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| cited_by | cdi_FETCH-LOGICAL-c390t-e526c2d4e3db36b41d642a9019f3319767d1ac22109e1567a9fce07ef072e1213 |
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| container_end_page | 145 |
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| container_start_page | 135 |
| container_title | Acta biomaterialia |
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| creator | Bothe, Friederike Lotz, Benedict Seebach, Elisabeth Fischer, Jennifer Hesse, Eliane Diederichs, Solvig Richter, Wiltrud |
| description | [Display omitted]
Bioactive functional scaffolds are essential for support of cell-based strategies to improve bone regeneration. Adipose-tissue-derived-stromal cells (ASC) are more accessible multipotent cells with faster proliferation than bone-marrow-derived-stromal-cells (BMSC) having potential to replace BMSC for therapeutic stimulation of bone-defect healing. Their osteogenic potential is, however, lower compared to BMSC, a deficit that may be overcome in growth factor-rich orthotopic bone defects with enhanced bone-conductive scaffolds. Objective of this study was to compare the therapeutic potency of human ASC and BMSC for bone regeneration on a novel nanoparticulate β-TCP/collagen-carrier (β-TNC).
Cytotoxicity of β-TCP nanoparticles and multilineage differentiation of cells were characterized in vitro. Cell-seeded β-TNC versus cell-free controls were implanted into 4 mm calvarial bone-defects in immunodeficient mice and bone healing was quantified by µCT at 4 and 8 weeks. Tissue-quality and cell-origin were assessed by histology.
β-TNC was non-toxic, radiolucent and biocompatible, lent excellent support for human cell persistence and allowed formation of human bone tissue by BMSC but not ASC. Opposite to BMSC, ASC-grafting significantly inhibited calvarial bone healing compared to controls. Bone formation progressed significantly from 4 to 8 weeks only in BMSC and controls yielding 5.6-fold more mineralized tissue in BMSC versus ASC-treated defects.
Conclusively, β-TNC was simple to generate, biocompatible, osteoconductive, and stimulated osteogenicity of BMSC to enhance calvarial defect healing while ASC had negative effects. Thus, an orthotopic environment and β-TNC could not compensate for cell-autonomous deficits of ASC which should systematically be considered when choosing the right cell source for tissue engineering-based stimulation of bone regeneration.
Bone-marrow-derived-stromal cells (BMSC) implanted on bone replacement materials can support bone defect healing and adipose-tissue-derived-stromal cells (ASC) being more accessible and better proliferating are considered as alternate source. This first standardized comparison of the bone regeneration potency of human ASC and BMSC was performed on a novel nanoparticular β-TCP-enriched collagen-carrier (β-TNC) designed to overcome the known inferior osteogenicity of ASC. β-TNC was non-toxic, biocompatible and osteoconductive supporting human bone formation and defect-closure by BMSC but not |
| doi_str_mv | 10.1016/j.actbio.2018.06.026 |
| format | article |
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Bioactive functional scaffolds are essential for support of cell-based strategies to improve bone regeneration. Adipose-tissue-derived-stromal cells (ASC) are more accessible multipotent cells with faster proliferation than bone-marrow-derived-stromal-cells (BMSC) having potential to replace BMSC for therapeutic stimulation of bone-defect healing. Their osteogenic potential is, however, lower compared to BMSC, a deficit that may be overcome in growth factor-rich orthotopic bone defects with enhanced bone-conductive scaffolds. Objective of this study was to compare the therapeutic potency of human ASC and BMSC for bone regeneration on a novel nanoparticulate β-TCP/collagen-carrier (β-TNC).
Cytotoxicity of β-TCP nanoparticles and multilineage differentiation of cells were characterized in vitro. Cell-seeded β-TNC versus cell-free controls were implanted into 4 mm calvarial bone-defects in immunodeficient mice and bone healing was quantified by µCT at 4 and 8 weeks. Tissue-quality and cell-origin were assessed by histology.
β-TNC was non-toxic, radiolucent and biocompatible, lent excellent support for human cell persistence and allowed formation of human bone tissue by BMSC but not ASC. Opposite to BMSC, ASC-grafting significantly inhibited calvarial bone healing compared to controls. Bone formation progressed significantly from 4 to 8 weeks only in BMSC and controls yielding 5.6-fold more mineralized tissue in BMSC versus ASC-treated defects.
Conclusively, β-TNC was simple to generate, biocompatible, osteoconductive, and stimulated osteogenicity of BMSC to enhance calvarial defect healing while ASC had negative effects. Thus, an orthotopic environment and β-TNC could not compensate for cell-autonomous deficits of ASC which should systematically be considered when choosing the right cell source for tissue engineering-based stimulation of bone regeneration.
Bone-marrow-derived-stromal cells (BMSC) implanted on bone replacement materials can support bone defect healing and adipose-tissue-derived-stromal cells (ASC) being more accessible and better proliferating are considered as alternate source. This first standardized comparison of the bone regeneration potency of human ASC and BMSC was performed on a novel nanoparticular β-TCP-enriched collagen-carrier (β-TNC) designed to overcome the known inferior osteogenicity of ASC. β-TNC was non-toxic, biocompatible and osteoconductive supporting human bone formation and defect-closure by BMSC but not ASC. Long-term cell-persistence and the distinct secretome of ASC appear as main reasons why ASC inhibited bone healing opposite to BMSC. Overall, ASC-grafting is at considerable risk of producing negative effects on bone-healing while no such risks are known for BMSC.</description><identifier>ISSN: 1742-7061</identifier><identifier>EISSN: 1878-7568</identifier><identifier>DOI: 10.1016/j.actbio.2018.06.026</identifier><identifier>PMID: 29933108</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Accessibility ; Adipose Tissue - metabolism ; Adipose Tissue - pathology ; Adipose tissue-derived stromal cells ; Animals ; Biocompatibility ; Biomedical materials ; Bone growth ; Bone healing ; Bone marrow ; Bone Marrow Cells - metabolism ; Bone Marrow Cells - pathology ; Bone-marrow-derived mesenchymal stromal cells ; Calcium Phosphates - chemistry ; Calcium Phosphates - pharmacology ; Calvarial bone regeneration ; Cell growth ; Cell proliferation ; Collagen ; Cytotoxicity ; Defects ; Differentiation (biology) ; Environmental effects ; Female ; Fracture Healing ; Grafting ; Growth factors ; Healing ; Histology ; Humans ; Immunodeficiency ; Mice ; Mice, SCID ; Nanoparticles ; Nanoparticles - chemistry ; Nanoparticles - therapeutic use ; Osteoconduction ; Osteogenesis ; Quality assessment ; Regeneration ; Regeneration (physiology) ; Scaffolds ; Secretome ; Skull - injuries ; Skull - metabolism ; Skull - pathology ; Stimulation ; Stromal cells ; Stromal Cells - metabolism ; Stromal Cells - pathology ; Stromal Cells - transplantation ; Tissue engineering ; Toxicity ; Wound healing ; β-TCP</subject><ispartof>Acta biomaterialia, 2018-08, Vol.76, p.135-145</ispartof><rights>2018 Acta Materialia Inc.</rights><rights>Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Aug 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-e526c2d4e3db36b41d642a9019f3319767d1ac22109e1567a9fce07ef072e1213</citedby><cites>FETCH-LOGICAL-c390t-e526c2d4e3db36b41d642a9019f3319767d1ac22109e1567a9fce07ef072e1213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29933108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bothe, Friederike</creatorcontrib><creatorcontrib>Lotz, Benedict</creatorcontrib><creatorcontrib>Seebach, Elisabeth</creatorcontrib><creatorcontrib>Fischer, Jennifer</creatorcontrib><creatorcontrib>Hesse, Eliane</creatorcontrib><creatorcontrib>Diederichs, Solvig</creatorcontrib><creatorcontrib>Richter, Wiltrud</creatorcontrib><title>Stimulation of calvarial bone healing with human bone marrow stromal cells versus inhibition with adipose-tissue stromal cells on nanostructured β-TCP-collagen</title><title>Acta biomaterialia</title><addtitle>Acta Biomater</addtitle><description>[Display omitted]
Bioactive functional scaffolds are essential for support of cell-based strategies to improve bone regeneration. Adipose-tissue-derived-stromal cells (ASC) are more accessible multipotent cells with faster proliferation than bone-marrow-derived-stromal-cells (BMSC) having potential to replace BMSC for therapeutic stimulation of bone-defect healing. Their osteogenic potential is, however, lower compared to BMSC, a deficit that may be overcome in growth factor-rich orthotopic bone defects with enhanced bone-conductive scaffolds. Objective of this study was to compare the therapeutic potency of human ASC and BMSC for bone regeneration on a novel nanoparticulate β-TCP/collagen-carrier (β-TNC).
Cytotoxicity of β-TCP nanoparticles and multilineage differentiation of cells were characterized in vitro. Cell-seeded β-TNC versus cell-free controls were implanted into 4 mm calvarial bone-defects in immunodeficient mice and bone healing was quantified by µCT at 4 and 8 weeks. Tissue-quality and cell-origin were assessed by histology.
β-TNC was non-toxic, radiolucent and biocompatible, lent excellent support for human cell persistence and allowed formation of human bone tissue by BMSC but not ASC. Opposite to BMSC, ASC-grafting significantly inhibited calvarial bone healing compared to controls. Bone formation progressed significantly from 4 to 8 weeks only in BMSC and controls yielding 5.6-fold more mineralized tissue in BMSC versus ASC-treated defects.
Conclusively, β-TNC was simple to generate, biocompatible, osteoconductive, and stimulated osteogenicity of BMSC to enhance calvarial defect healing while ASC had negative effects. Thus, an orthotopic environment and β-TNC could not compensate for cell-autonomous deficits of ASC which should systematically be considered when choosing the right cell source for tissue engineering-based stimulation of bone regeneration.
Bone-marrow-derived-stromal cells (BMSC) implanted on bone replacement materials can support bone defect healing and adipose-tissue-derived-stromal cells (ASC) being more accessible and better proliferating are considered as alternate source. This first standardized comparison of the bone regeneration potency of human ASC and BMSC was performed on a novel nanoparticular β-TCP-enriched collagen-carrier (β-TNC) designed to overcome the known inferior osteogenicity of ASC. β-TNC was non-toxic, biocompatible and osteoconductive supporting human bone formation and defect-closure by BMSC but not ASC. Long-term cell-persistence and the distinct secretome of ASC appear as main reasons why ASC inhibited bone healing opposite to BMSC. Overall, ASC-grafting is at considerable risk of producing negative effects on bone-healing while no such risks are known for BMSC.</description><subject>Accessibility</subject><subject>Adipose Tissue - metabolism</subject><subject>Adipose Tissue - pathology</subject><subject>Adipose tissue-derived stromal cells</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Bone Marrow Cells - pathology</subject><subject>Bone-marrow-derived mesenchymal stromal cells</subject><subject>Calcium Phosphates - chemistry</subject><subject>Calcium Phosphates - pharmacology</subject><subject>Calvarial bone regeneration</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Collagen</subject><subject>Cytotoxicity</subject><subject>Defects</subject><subject>Differentiation (biology)</subject><subject>Environmental effects</subject><subject>Female</subject><subject>Fracture Healing</subject><subject>Grafting</subject><subject>Growth factors</subject><subject>Healing</subject><subject>Histology</subject><subject>Humans</subject><subject>Immunodeficiency</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - therapeutic use</subject><subject>Osteoconduction</subject><subject>Osteogenesis</subject><subject>Quality assessment</subject><subject>Regeneration</subject><subject>Regeneration (physiology)</subject><subject>Scaffolds</subject><subject>Secretome</subject><subject>Skull - injuries</subject><subject>Skull - metabolism</subject><subject>Skull - pathology</subject><subject>Stimulation</subject><subject>Stromal cells</subject><subject>Stromal Cells - metabolism</subject><subject>Stromal Cells - pathology</subject><subject>Stromal Cells - transplantation</subject><subject>Tissue engineering</subject><subject>Toxicity</subject><subject>Wound healing</subject><subject>β-TCP</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kc9u1DAQxiMEoqXwBghZ4sIl6dhJ7OSChFZAK1UqEuVsOc6k61ViL_6zFW_DM_AgPBPepnDogdNYo9_3zXi-onhNoaJA-fmuUjoOxlUMaFcBr4DxJ8Up7URXipZ3T_NbNKwUwOlJ8SKEHUDdUdY9L05Y39c1he60-Pk1miXNKhpniZuIVvNBeaNmMjiLZItqNvaW3Jm4Jdu0KLv2F-W9uyMherdkVuM8B3JAH1Igxm7NYO4N72VqNHsXsIwmhISPNBmyyrrcTDomjyP5_au82XwptZtndYv2ZfFsUnPAVw_1rPj26ePN5qK8uv58uflwVeq6h1hiy7hmY4P1ONR8aOjIG6Z6oP2Uf9oLLkaqNGMUeqQtF6qfNILACQRDymh9VrxbfffefU8YolxMOO6oLLoUJIO2a6GrG57Rt4_QnUve5u0ko7RhDBrWZKpZKe1dCB4nufcm3-2HpCCPCcqdXBOUxwQlcJkTzLI3D-ZpWHD8J_obWQberwDmaxwMehm0QatxNB51lKMz_5_wB7ODsds</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Bothe, Friederike</creator><creator>Lotz, Benedict</creator><creator>Seebach, Elisabeth</creator><creator>Fischer, Jennifer</creator><creator>Hesse, Eliane</creator><creator>Diederichs, Solvig</creator><creator>Richter, Wiltrud</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201808</creationdate><title>Stimulation of calvarial bone healing with human bone marrow stromal cells versus inhibition with adipose-tissue stromal cells on nanostructured β-TCP-collagen</title><author>Bothe, Friederike ; Lotz, Benedict ; Seebach, Elisabeth ; Fischer, Jennifer ; Hesse, Eliane ; Diederichs, Solvig ; Richter, Wiltrud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-e526c2d4e3db36b41d642a9019f3319767d1ac22109e1567a9fce07ef072e1213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Accessibility</topic><topic>Adipose Tissue - metabolism</topic><topic>Adipose Tissue - pathology</topic><topic>Adipose tissue-derived stromal cells</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Bone growth</topic><topic>Bone healing</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Bone Marrow Cells - pathology</topic><topic>Bone-marrow-derived mesenchymal stromal cells</topic><topic>Calcium Phosphates - chemistry</topic><topic>Calcium Phosphates - pharmacology</topic><topic>Calvarial bone regeneration</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Collagen</topic><topic>Cytotoxicity</topic><topic>Defects</topic><topic>Differentiation (biology)</topic><topic>Environmental effects</topic><topic>Female</topic><topic>Fracture Healing</topic><topic>Grafting</topic><topic>Growth factors</topic><topic>Healing</topic><topic>Histology</topic><topic>Humans</topic><topic>Immunodeficiency</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - therapeutic use</topic><topic>Osteoconduction</topic><topic>Osteogenesis</topic><topic>Quality assessment</topic><topic>Regeneration</topic><topic>Regeneration (physiology)</topic><topic>Scaffolds</topic><topic>Secretome</topic><topic>Skull - injuries</topic><topic>Skull - metabolism</topic><topic>Skull - pathology</topic><topic>Stimulation</topic><topic>Stromal cells</topic><topic>Stromal Cells - metabolism</topic><topic>Stromal Cells - pathology</topic><topic>Stromal Cells - transplantation</topic><topic>Tissue engineering</topic><topic>Toxicity</topic><topic>Wound healing</topic><topic>β-TCP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bothe, Friederike</creatorcontrib><creatorcontrib>Lotz, Benedict</creatorcontrib><creatorcontrib>Seebach, Elisabeth</creatorcontrib><creatorcontrib>Fischer, Jennifer</creatorcontrib><creatorcontrib>Hesse, Eliane</creatorcontrib><creatorcontrib>Diederichs, Solvig</creatorcontrib><creatorcontrib>Richter, Wiltrud</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biomaterialia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bothe, Friederike</au><au>Lotz, Benedict</au><au>Seebach, Elisabeth</au><au>Fischer, Jennifer</au><au>Hesse, Eliane</au><au>Diederichs, Solvig</au><au>Richter, Wiltrud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulation of calvarial bone healing with human bone marrow stromal cells versus inhibition with adipose-tissue stromal cells on nanostructured β-TCP-collagen</atitle><jtitle>Acta biomaterialia</jtitle><addtitle>Acta Biomater</addtitle><date>2018-08</date><risdate>2018</risdate><volume>76</volume><spage>135</spage><epage>145</epage><pages>135-145</pages><issn>1742-7061</issn><eissn>1878-7568</eissn><abstract>[Display omitted]
Bioactive functional scaffolds are essential for support of cell-based strategies to improve bone regeneration. Adipose-tissue-derived-stromal cells (ASC) are more accessible multipotent cells with faster proliferation than bone-marrow-derived-stromal-cells (BMSC) having potential to replace BMSC for therapeutic stimulation of bone-defect healing. Their osteogenic potential is, however, lower compared to BMSC, a deficit that may be overcome in growth factor-rich orthotopic bone defects with enhanced bone-conductive scaffolds. Objective of this study was to compare the therapeutic potency of human ASC and BMSC for bone regeneration on a novel nanoparticulate β-TCP/collagen-carrier (β-TNC).
Cytotoxicity of β-TCP nanoparticles and multilineage differentiation of cells were characterized in vitro. Cell-seeded β-TNC versus cell-free controls were implanted into 4 mm calvarial bone-defects in immunodeficient mice and bone healing was quantified by µCT at 4 and 8 weeks. Tissue-quality and cell-origin were assessed by histology.
β-TNC was non-toxic, radiolucent and biocompatible, lent excellent support for human cell persistence and allowed formation of human bone tissue by BMSC but not ASC. Opposite to BMSC, ASC-grafting significantly inhibited calvarial bone healing compared to controls. Bone formation progressed significantly from 4 to 8 weeks only in BMSC and controls yielding 5.6-fold more mineralized tissue in BMSC versus ASC-treated defects.
Conclusively, β-TNC was simple to generate, biocompatible, osteoconductive, and stimulated osteogenicity of BMSC to enhance calvarial defect healing while ASC had negative effects. Thus, an orthotopic environment and β-TNC could not compensate for cell-autonomous deficits of ASC which should systematically be considered when choosing the right cell source for tissue engineering-based stimulation of bone regeneration.
Bone-marrow-derived-stromal cells (BMSC) implanted on bone replacement materials can support bone defect healing and adipose-tissue-derived-stromal cells (ASC) being more accessible and better proliferating are considered as alternate source. This first standardized comparison of the bone regeneration potency of human ASC and BMSC was performed on a novel nanoparticular β-TCP-enriched collagen-carrier (β-TNC) designed to overcome the known inferior osteogenicity of ASC. β-TNC was non-toxic, biocompatible and osteoconductive supporting human bone formation and defect-closure by BMSC but not ASC. Long-term cell-persistence and the distinct secretome of ASC appear as main reasons why ASC inhibited bone healing opposite to BMSC. Overall, ASC-grafting is at considerable risk of producing negative effects on bone-healing while no such risks are known for BMSC.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29933108</pmid><doi>10.1016/j.actbio.2018.06.026</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1742-7061 |
| ispartof | Acta biomaterialia, 2018-08, Vol.76, p.135-145 |
| issn | 1742-7061 1878-7568 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2058508346 |
| source | ScienceDirect Journals |
| subjects | Accessibility Adipose Tissue - metabolism Adipose Tissue - pathology Adipose tissue-derived stromal cells Animals Biocompatibility Biomedical materials Bone growth Bone healing Bone marrow Bone Marrow Cells - metabolism Bone Marrow Cells - pathology Bone-marrow-derived mesenchymal stromal cells Calcium Phosphates - chemistry Calcium Phosphates - pharmacology Calvarial bone regeneration Cell growth Cell proliferation Collagen Cytotoxicity Defects Differentiation (biology) Environmental effects Female Fracture Healing Grafting Growth factors Healing Histology Humans Immunodeficiency Mice Mice, SCID Nanoparticles Nanoparticles - chemistry Nanoparticles - therapeutic use Osteoconduction Osteogenesis Quality assessment Regeneration Regeneration (physiology) Scaffolds Secretome Skull - injuries Skull - metabolism Skull - pathology Stimulation Stromal cells Stromal Cells - metabolism Stromal Cells - pathology Stromal Cells - transplantation Tissue engineering Toxicity Wound healing β-TCP |
| title | Stimulation of calvarial bone healing with human bone marrow stromal cells versus inhibition with adipose-tissue stromal cells on nanostructured β-TCP-collagen |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T01%3A19%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stimulation%20of%20calvarial%20bone%20healing%20with%20human%20bone%20marrow%20stromal%20cells%20versus%20inhibition%20with%20adipose-tissue%20stromal%20cells%20on%20nanostructured%20%CE%B2-TCP-collagen&rft.jtitle=Acta%20biomaterialia&rft.au=Bothe,%20Friederike&rft.date=2018-08&rft.volume=76&rft.spage=135&rft.epage=145&rft.pages=135-145&rft.issn=1742-7061&rft.eissn=1878-7568&rft_id=info:doi/10.1016/j.actbio.2018.06.026&rft_dat=%3Cproquest_cross%3E2114220424%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c390t-e526c2d4e3db36b41d642a9019f3319767d1ac22109e1567a9fce07ef072e1213%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2114220424&rft_id=info:pmid/29933108&rfr_iscdi=true |